Tumor-derived exosomes (TEXs) contain enriched miRNAs that act as novel non-invasive biomarkers for cancer diagnosis and play a role in cancer progression. We investigated the exosomal miRNAs that affect cancer progression in non-small cell lung cancer (NSCLC) and identified the specific molecules involved. We identified that specific miRNAs in NSCLC cell-released exosomes can modulate angiogenesis, among which miR-619-5p was the most potent inducer. RCAN1.4 was identified as a target of miR-619-5p and its suppression promoted angiogenesis. Furthermore, the suppression of RCAN1.4 induced cell proliferation and metastasis in NSCLC cells. In patients with NSCLC, the level of RCAN1.4 expression was significantly lower, and that of miR-619-5p significantly higher, in tumor than normal lung tissues. miR-619-5p expression was higher than normal in exosomes isolated from the plasma of NSCLC patients. Finally, hypoxic conditions induced miR-619-5p upload into NSCLC cell-derived exosomes. Our findings indicate that exosomal miR-619-5p promotes the growth and metastasis of NSCLCs by regulating RCAN1.4 and can serve as a diagnostic indicator for these lung cancers.
(F) HUVECs were treated with 50 µg of exosomes derived from A549, H460 and BEAS-2B cells. Effect of exosomes on the tube formation ability of HUVECs, as determined by a tube formation assay. Credit: Dong et. al., doi.org/10.1016/j.canlet.2020.01.023
In this study, the culture insert was used to determine the migration and demonstrate that the exosomes released from non-small cell lung cancer (NSCLC) cells (A549, H460) enhance the angiogenic behavior of endothelial cells in vitro.
Learn more about culture-insert for wound healing and migration assays.