The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. Combine with Azure Transfer Buffer (AC2127) to cut wet transfer time down to 15-to 30-minutes for a total assay time of two hours from gel to imaging
Rapid protocol with low background and high sensitivity
Replicate Western blots were performed using the Azure Flash Western Chemiluminescence kit and a traditional Western blot protocol. Each blot shows a dilution series of the same sample. The Azure Flash Western kit produced more sensitive detection than the traditional protocol with over 90% of antibodies (while STAT-3 and pSTAT-3 demonstrate the improved data quality possible with Azure Flash Western Kit, the HSP60 antibody is an example of an antibody with similar performance).
Compare to a traditional Western blot protocol, the Azure Flash Western can take three days of work down to less than 2 hours.