131.00 USD – 392.00 USD
A safe, ready-to-use luminescent protein gel stain. Because it requires no fixation or wash steps, it is a big improvement on the original Lumitein™ protein gel stain.
One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain in terms of convenience and safety. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band.
One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation. Destaining is optional. One-Step Lumitein™ can detect as little as approximately 1-10 ng of protein per band depending on the staining method used, although staining intensity varies between proteins. Staining is fully compatible with mass spectrometry and Edman-based sequencing. Staining can be imaged using a UV transilluminator with EtBr or SYBR filter set, a blue light transilluminator, or a laser-based gel scanner.
One-Step Lumitein™ Protein Gel Stain offers safer handling and ease of disposal compared to other protein gel stains, because it is an aqueous-based solution that does not contain hazardous methanol or acetic acid. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step. Furthermore, One-Step Lumitein™ costs significantly less than other fluorescent protein gel stains. One-Step Lumitein™ Protein Gel Stain is available in 1 liter bottle or 4 liter Cubitainer® sizes.
Please visit our Protein Gel Stains technology page to learn about our other safe and sensitive protein gel stains such as One-Step Lumitein™ UV, which is optimized for use with a UV transilluminator, and One-Step Blue™, a rapid, easy-to-use, non-toxic alternative to Coomassie staining for visible blue staining.
|Product||Replacement for||Catalog number||Unit size||Product protocol||Safety report|
|One-Step Blue®||Coomassie stain||21003-1L||1L||PI-21003||One-Step Gel Stains Safety Report|
|One-Step Lumitein™||SYPRO® Ruby Protein Gel Stain||21004-1L||1L||PI-21004|
|One-Step Lumitein™ UV||Oriole™ and Flamingo™ Protein Gel Stains||21005-1L||1L||PI-21005|
|Excitation/Emission||280, 460 (broad)/610 nm|