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IFN alpha 1a, Human

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SKU: C01078 Category: Tags: , ,

Description

Interferon alpha-1 or interferon alpha-D, encoded by the human IFNA1gene, is a member of the type I interferon family . It is expressed in peripheral blood leukocytes and lymphoblastoid cells. There are four highly conserved cysteine residues which form two disulfide bonds. One bond is a necessity for biological activity, which plays an critical role in conducting non-specific resistance against a variety of viral infections, and regulation of cell proliferation and modulates immune responses.

Sequence: 
CDLPETHSLDNRRTLMLLAQMSRISPSSCLMDRHDFGFPQEEFDGNQFQKAPAISVLHELIQQIFNLFTTKDSSAAWDEDLLDKFCTELYQQ
LNDLEACVMQEERVGETPLMNADSILAVKKYFRRITLYLTEKKYSPCAWEVVRAEIMRSLSLSTNLQERLRRKE with polyhistidine tag at
the N-terminus

Source:
Escherichia coli

Endotoxin Test:
<0.1 EU per 1 μg of the protein by the LAL method.

Activity:
Measure by its ability to inhibit IL-8 secretion in human PBMCs in the presence of LPS. The ED50 for this effect is <1.12 μg /mL.

Purity:
>95% as determined by SDS-PAGE. Ni-NTA chromatography

Formulation:
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

Reconstitution:
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

Storage:
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

Note:
Please use within one month after protein reconstitution.



 

Additional information

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5 μg, 20 μg, 100 µg

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