96.00 USD – 475.20 USD
FasL is a member of the TNF superfamily, and is mainly expressed on the cell surface of activated T cells. FasL induces apoptosis in Fas-bearing cells by binding to Fas Receptor. FasL has the ability to leads to down-regulation of the immune response through killing T cells and activated B cells. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death.
VMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL with polyhistidine tag and sumo tag at the N-terminus
<0.1 EU per 1 μg of the protein by the LAL method.
Measure by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 106 IU/mg.
>98% as determined by SDS-PAGE. Ni-NTA chromatography
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Please use within one month after protein reconstitution.
5 μg, 20 μg, 100 µg