66.00 USD – 287.00 USD
A high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
2X Forget-Me-Not™ Universal qPCR Probe Master Mix is a high-performance product for fluorescent probe-based PCR applications, including quantitation and SNP genotyping.
The master mix contains Cheetah™ HotStart Taq DNA Polymerase and dNTPs in a buffer optimized for high qPCR sensitivity and multiplex reactions. Only primers, probe, and template need to be added.
Biotium’s proprietary chemically modified hot-start DNA Polymerase. Cheetah™ Taq is completely inactive at room temperature, but is fully activated in 2 minutes at 95°C with high activity recovery, making it particularly suitable for fast PCR.
The 40X Forget-Me-Not™ Tracking Buffer contains an inert blue dye. You have the choice of adding Tacking Buffer to the master mix, to the DNA template, or not to use the tracking buffer in your reactions. Addition of Tracking Buffer to your master mix allows you to easily distinguish wells containing reaction mix from empty wells. Alternatively, when added to your DNA template samples, the Tracking Buffer allows you to see which reactions have had template added while you set up your PCR reactions.
|Select reference dye||
No ROX, With Separate ROX
1 mL (100 reactions) Trial Size, 5 x 1 mL (500 reactions)