38.00 USD – 2,549.00 USD
GelGreen® is a sensitive, non-mutagenic and environmentally safer green fluorescent DNA gel stain
GelGreen® is a highly sensitive, non-toxic green fluorescent nucleic acid dye designed for staining DNA in agarose gels.
GelGreen® is far more sensitive than SYBR® Safe. Unlike SYBR® dyes, which are known to be unstable, GelGreen® is very stable, both hydrolytically and thermally. GelGreen® is compatible 254 nm UV transilluminator, and can be imaged using a SYBR® Green or GelStar® filter. It also can be used with visible blue light excitation imagers (blue LED light box or Dark Reader®). With blue light illuminators, researchers can avoid exposure to UV irradiation for themselves and their DNA samples, for a safer work environment and higher cloning efficiency.
A series of safety tests have confirmed that GelGreen® is noncytotoxic, nonmutagenic and nonhazardous at concentrations above those used for gel staining. As a result, working strength GelGreen® can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed®/GelGreen® Safety Report.
Many so-called “safe” DNA dyes like SYBR® Safe, Midori Green, GreenSafe, SafeView™, and RedSafe™ not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic. Unlike these dyes, GelGreen® is cell membrane-impermeant, so it cannot enter living cells to interact with their DNA. See our Gel Stains Comparison Flyer or Gel Stains Comparison White Paper for details.
For new users, we recommend GelGreen® 10,000X in water (catalog no. 41005), our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelGreen® 10,000X solution in DMSO for established users who do not wish to change their existing laboratory protocols. We also offer GelGreen® Agarose for convenient and safer preparation of precast gels. Also see GelRed® Nucleic Acid Gel Stain, our safer replacement for ethidium bromide.
GelGreen® can be used to stain ssDNA and RNA, but we recommend GelRed® for this application because it is five times more sensitive for single stranded nucleic acids than GelGreen®.
Download a list of selected References for GelRed® and GelGreen®.
41005, 41005-1, 41005-T
0.1mL, 0.5mL, 10 mL