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AccuGreen™ High Sensitivity dsDNA Quantitation Kit

119.00 USD333.00 USD

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Description

A fluorescent dsDNA quantitation kit with a linear range of 0.1-100 ng DNA. Designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher Scientific.

Product Description

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples.  The kit provides a linear detection range from 0.1 – 100 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific. The AccuGreen™ High Sensitivity assay is also available without a DNA standard (31068), for those that wish to use their own standard.

Features

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 0.1-100 ng dsDNA
  • Sample concentration range: 5 pg/uL to 100 ng/uL
  • Green fluorescence emission
  • Replaces Qubit® dsDNA HS Assay Kit for cost savings

Kit Components

  • AccuGreen™ Dye, 200X
  • DNA Quantitation Buffer, 20X Concentrate*
  • AccuGreen™ Standard 1 (0 ng/uL)
  • AccuGreen™ Standard 2 (10 ng/uL)

*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

AccuGreen™ for the Qubit® fluorometer

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific.

The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.

A genomic DNA sample quantified using the Qubit® HS kit and the AccuGreen™ HS kit. A nearly identical result was obtained with each kit.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA Quantitation Kits

dsDNA Quantitation Kit Linear detection range
(dsDNA in assay)
Ex/Em (nm) Suggested Instrument Features
AccuGreen™ High Sensitivity 0.1-100 ng 502/523 Qubit® fluorometer Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range 2-1000 ng 500/530 Qubit® fluorometer Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen 1 pg-3 ng* 468/507 Fluorescent Plate Reader Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity 30 pg-250 ng 468/507 Fluorescent Plate Reader Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity 0.2-100 ng 485/530 Fluorescent Plate Reader Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range 2-2000 ng 350/460 Fluorescent Plate Reader Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.

References

1. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102
2. J Forensic Sci (2018) 63;5 doi:10.1111/1556-4029.13837

Product Attributes

Excitation/Emission 502/523 nm (with DNA)
Storage Conditions Store at 2 to 8 °C

Protocols

SDS

Additional information

Size

100 assays, 500 assays

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