Description
An RNA-selective, fluorescent RNA quantitation kit with the widest linear range (5 ng–1000 ng RNA) of any RNA quantitation assay. Designed for use in a microplate reader or a Qubit® fluorometer.
Product Description
The AccuBlue® Broad Range RNA Quantitation Kit boasts the widest linear range (5 ng–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA.
Features
- Linear range (5-1000 ng RNA) spans Qubit® HS & BR ranges
- Highly selective for RNA over dsDNA
- Far-red fluorescence emission
- For fluorescence microplate reader or Qubit® fluorometer (RNA Broad Range program)
- Mammalian RNA standard to accurately quantify similar samples
Kit Components
- RNA Broad Range Dye, 200X
- RNA Broad Range Buffer
- RNA Dilution Buffer (can use as Standard 1 on Qubit®)
- RNA Broad Range Standard, 100 ng/uL* (can use as Standard 2 on Qubit®)
*Replacement vials of RNA standard (cat # 99850) can be purchased separately in case the RNA standard in your kit becomes degraded or otherwise compromised.
Assay range
The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit® HS and Qubit® BR ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).
RNA specificity
Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).
Instrument compatibility
The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimized for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.
Product Attributes
| Shipping condition | Ice pack |
|---|---|
| Storage Conditions | Store at 2 to 8 °C |
| Excitation/Emission | 650/670 nm |
Protocols
SDS
Supporting Documents
Additional information
| Size | Trial size (200 assays), 1000 assays |
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