92.00 USD – 311.00 USD
An RNA-selective, fluorescent RNA quantitation kit with the widest linear range (5 ng–1000 ng RNA) of any RNA quantitation assay. Designed for use in a microplate reader or a Qubit® fluorometer.
The AccuBlue® Broad Range RNA Quantitation Kit boasts the widest linear range (5 ng–1000 ng RNA) of available RNA quantitation kits, while providing exceptional accuracy, sensitivity, and high RNA selectivity. A mammalian RNA standard is included for accurate quantitation of mammalian cell RNA.
*Replacement vials of RNA standard (cat # 99850) can be purchased separately in case the RNA standard in your kit becomes degraded or otherwise compromised.
The assay is linear between 5 and 1000 ng of RNA per 200 uL assay well, which spans both Qubit® HS and Qubit® BR ranges. It is ideal for use in quantifying RNA for sensitive applications such as Next-Gen Sequencing (NGS) or reverse transcription PCR (RT-PCR).
Unlike absorbance-based measurements, RNA Broad Range Dye is selective for RNA over double-stranded DNA, and can tolerate an equimolar amount of dsDNA in the sample without significant effect on RNA quantitation (purified RNA samples are still recommended).
The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with excitation and emission filters for detecting far-red fluorescence. It is also optimized for use in the Qubit® fluorometer, using the pre-programmed RNA Broad Range program.
Trial size (200 assays), 1000 assays