47.00 USD – 180.00 USD
A fluorescent dsDNA quantitation solution with a linear range of 2-2000 ng DNA. Designed for use in a fluorescence microplate reader.
The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a linear detection range from 2 – 2000 ng and is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® Broad Range Kit (31007) which contains a set of calf thymus DNA standards.
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
|dsDNA Quantitation Kit||Linear detection range
(dsDNA in assay)
|Ex/Em (nm)||Suggested Instrument||Features|
|AccuGreen™ High Sensitivity||0.1-100 ng||502/523||Qubit® fluorometer||Compare to the Qubit® dsDNA HS assay from Thermo Fisher|
|AccuGreen™ Broad Range||2-1000 ng||500/530||Qubit® fluorometer||Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
|AccuBlue® NextGen||1 pg-3 ng*||468/507||Fluorescent Plate Reader||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
|AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Fluorescent Plate Reader||Versatile kit with high sensitivity and wide linear range|
|AccuBlue® High Sensitivity||0.2-100 ng||485/530||Fluorescent Plate Reader||Non-toxic & non-mutagentic DNA quantitation dye|
|AccuBlue® Broad Range||2-2000 ng||350/460||Fluorescent Plate Reader||Broad linear range with blue fluorescence|
1. PLoS ONE (2013), Dec 6;8(12) e80613 doi:10.1371/journal.pone.0080613
2. Am J Med Genet B Neuro Genet (2013) 162(B)(4)404-12 doi:10.1002/ajmg.b.32157
3. PLoS ONE (2015), Feb 6;10(2) e0117792 doi:10.1371/journal.pone.0117792
4. PLoS ONE (2015), Jul 6;10(7):e0132137 doi:10.1371/journal.pone.0132137
5. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102
|Excitation/Emission||350/460 nm (with DNA)|
|Storage Conditions||Store at 2 to 8 °C|
Trial size (200 assays), 1500 assays