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AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards

62.00 USD269.00 USD

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Description

A fluorescent dsDNA quantitation kit with a linear range of 2-2000 ng DNA. Designed for use in a fluorescence microplate reader.

Product Description

The AccuBlue® Broad Range dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The kit provides a linear detection range from 2 – 2000 ng and is designed for use with fluorescence microplate readers. The AccuBlue® Broad Range assay is also available without any DNA standards (31009), for those that wish to use their own standard.

Features

  • Highly specific for dsDNA
  • Broad linear detection range, from 2-2000 ng
  • Designed for fluorescence microplate readers
  • Blue fluorescence emission

Kit Components

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X
  • AccuBlue® dsDNA Standards, set of 9 (in 1000 assay kit)
  • AccuBlue® dsDNA Standard, 200 ng/uL (in trial size kit)

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA Quantitation Kits

dsDNA Quantitation Kit Linear detection range
(dsDNA in assay)
Ex/Em (nm) Suggested Instrument Features
AccuGreen™ High Sensitivity 0.1-100 ng 502/523 Qubit® fluorometer Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range 2-1000 ng 500/530 Qubit® fluorometer Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen 1 pg-3 ng* 468/507 Fluorescent Plate Reader Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity 30 pg-250 ng 468/507 Fluorescent Plate Reader Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity 0.2-100 ng 485/530 Fluorescent Plate Reader Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range 2-2000 ng 350/460 Fluorescent Plate Reader Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.

References

1. PLoS ONE (2013), Dec 6;8(12) e80613 doi:10.1371/journal.pone.0080613
2. Am J Med Genet B Neuro Genet (2013) 162(B)(4)404-12 doi:10.1002/ajmg.b.32157
3. PLoS ONE (2015), Feb 6;10(2) e0117792 doi:10.1371/journal.pone.0117792
4. PLoS ONE (2015), Jul 6;10(7):e0132137 doi:10.1371/journal.pone.0132137
5. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102

Product Attributes

Excitation/Emission 350/460 nm (with DNA)
Storage Conditions Store at 2 to 8 °C

Protocols

SDS

Additional information

Size

Trial size (200 assays), 1500 assays

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