Traditional on-chip droplet generation process requires extensive training in engineering and microfluidics in order to setup and maintain a multi-component system involving microscopic parts, in addition to devising and manufacturing a microfluidic chip. Furthermore, the process of generating droplets requires constant monitoring of the setup and eliminating potential external interferences, particularly statistics.
RAN Biotech is introducing the first commercial kit for Chip-Free Engineered Droplet Generation. The kit includes reagents which lead to the generation of droplets, monodispersed in size, without the need for microfluidic setup or microfluidic chips. Instead, vortexing or pipetting is all that is needed to generate a monodispersed emulsion.
Advantages of the Chip-Free Engineered Droplet Generation kit (NoChipDropTM kit):
•Production of monodispersed droplets in less than a minute without the need for specialized
•Droplets are generated using RAN Biotechnologies’ surfactants, gel beads and standard lab
equipment (vortex or pipette).
•Droplets are stable with various enzymatic formulations for applications such as PCR, cDNA
synthesis, ddPCR and single cell encapsulation.
•High throughput emulsification of multiple samples in microcentrifuge tubes or standard well
•Droplets can easily be imaged and aqueous phases can easily be recovered.
•Custom functionalization is available for gel beads (size, surface modifications
Different kinds of NoChipDrop Kits :
1. NoChipDrop kit 1
- 0.35mL of Bead slurry (ca. 1.27M, 58um beads)
- 0.5mL of emulsion oil
- 0.125mL of aqueous recovery oil
2. NoChipDrop Kit A
- 1.4mL of bead slurry (ca. 5.08M, 58um beads)
- 2.2mL of emulsion oil
- 0.55mL of aqueous recovery oil
3. NoChipDrop Kit B
- 7mL of bead slurry (ca. 25.4M, 58um beads)
- 11mL of emulsion oil
- 2.75mL of aqueous recovery oil