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PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2) – 200 assays

359.00 USD

Description

A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

Product Description

This kit is designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.

Features

  • Selectively detect viable Mycobacterium tuberculosis using qPCR
  • Contains primers to amplify the groEL2 gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA
  • Sufficient reagents for 200 PCR reactions

Kit components

  • PMA dye
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • groEL2 primer mix, 5 uM

About this kit

Mycobacterium tuberculosis is a pathogenic bacteria that infects the lungs and causes the disease tuberculosis. PCR to detect Mycobacterium tuberculosis has been reported using the primers provided in the kit. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note: groEL2 primers also amplify other mycobacteria species, but products may be distinguishable by melt curve analysis.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria Strain Gene name Kit with PMA (catalog #) Kit with PMAxx™ (catalog #)
Salmonella enterica invA 31033 31033-X
Mycobacterium tuberculosis groEL2 31034 N/A
Staphylococcus aureus nuc 31035 N/A
MRSA mecA 31036 N/A
E. coli O157:H7 Z3276 31037 31037-X
E. coli uidA 31050 31050-X
Listeria monocytogenes hly 31051 31051-X
Legionella pneumophila mip 31053 N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

Product Attributes

Viability dye PMA
Storage Conditions Store at -10 to -35 °C

Protocols