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Ghost Dye™ UV 450

66.00 USD255.00 USD

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Description

       

Ghost Dye™ UV 450 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Ghost Dye™ UV 450 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.

Name Ghost Dye™ UV 450
Cat. No. 13-0868
Protocol Download Protocol
Excitation Laser UV (355 nm)
Emission (nm) 450
Formulation 1 µL/test in DMSO
Application Flow Cytometry

Product Details

FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.

 

 

Recent Publications:

Marcia de Almeida Monteiro Melo Ferraz, Mayako Fujihara, Jennifer Beth Nagashima, Michael James Noonan, Miho Inoue-Murayama, Nucharin Songsasen. Follicular Extracellular Vesicles Enhance Meiotic Resumption of Domestic Cat Vitrified Oocytes. Sci Rep. 2020 May 25;10(1):8619. doi: 10.1038/s41598-020-65497-w.

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Derek W. Trobaugh, Chengqun Sun, Matthew D. Dunn, Douglas S. Reed, William B. Klimstra. Rational design of a live-attenuated eastern equine encephalitis virus vaccine through informed mutation of virulence determinants. PLoS Pathog. 2019 Feb 11;15(2):e1007584. doi: 10.1371/journal.ppat.1007584
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Jessica K. Fiege, Ian A. Stone, Elizabeth J. Fay, Matthew W. Markman, Sathi Wijeyesinghe, Marissa G. Macchietto, Steven Shen, David Masopust and Ryan A. Langlois. The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection. J Immunol. 2019 Aug 15;203(4):936-945. doi: 10.4049/jimmunol.1900093
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Jessica K. Fiege, Ian A. Stone, Rebekah E. Dumm, Barbara M. Waring, Brian T. FifeI, Judith Agudo, Brian D. Brown, Nicholas S. Heaton, Ryan A. Langlois. Long-term surviving influenza infected cells evade CD8+ T cell mediated clearance. PLoS Pathog. 2019 Sep 26;15(9):e1008077. doi: 10.1371/journal.ppat.1008077
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Additional information

Cat. No

13-0868-T100, 13-0868-T500

No. of Tests

100 tests, 500 Tests

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No. of Tests

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