The FGK45 antibody reacts with mouse CD40, a 40-50 kDa glycoprotein member of the tumor necrosis factor receptor (TNFR) family. CD40 is constitutively expressed by antigen presenting cells, including dendritic cells, B cells, and macrophages. The interaction of CD40 and its ligand CD154, expressed on activated T cells, plays an essential role in mediating a broad range of immune and inflammatory responses including T cell dependent immunoglobulin class switching, germinal center formation, and the development of memory B cells.
The FGK45 clone is reported to be functionally useful for both in vitro and in vivo activation of CD40 expressing antigen presenting cells.
||In Vivo Ready™ Anti-Mouse CD40 (FGK45)
||Bp50, gp39, HIGM1, IGM, IMD3, T-BAM, TRAP, TNFRSF5, p50, FGK4.5
||In Vivo Ready™
||Flow Cytometry, Functional Assays
||Shepherd DM, Kerkvliet NI. 1999. J Immunol. Sep 1;163(5):2470-2477.
Mauri C, Mars LT, Londei M. 2000. Nat Med. Jun;6(6):673-679.
Hellings PW, Kasran A, Bullens D, Overbergh L, Mathieu C, Heremans H, Matthys P, Boon L, Jorissen M, Ceuppens JL. 2006. J Immunol. Oct 15;177(8):5138-5144.
Bartholdy C, Kauffmann SO, Christensen JP, Thomsen AR. 2007. J Immunol. Feb 1;178(3):1662-1670.
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
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