The 2.4G2 antibody is specific for a common epitope found in the extracellular regions of mouse Fc-receptors Fc-gamma II (CD32) and Fc-gamma III (CD16). As these are receptors for the Fc portion of mouse IgG, they may also bind laboratory antibody preparations and products used in a variety of cell analysis protocols such as flow cytometry, immunohistochemistry and functional cell assays. The 2.4G2 antibody is therefore widely used as a pre-treatment reagent to block binding of specific antibodies of interest, e.g. fluorescently conjugated antibodies, to Fc receptors via their Fc domains and contributing to “non-specific” staining.
|Name||In Vivo Ready™ Anti-Mouse CD16 / CD32 (Fc Shield) (2.4G2)|
|Alternative Names||FCGR3, IGFR3; FCGR2, IGFR2; FC Receptor|
|Format||In Vivo Ready™|
|Application||Flow Cytometry, Functional Assays, IF, IP|
|Citations*||Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Blocking- Immunofluorescence microscopy)
Yamaji O, Nagaishi T, Totsuka T, Onizawa M, Suzuki M, Tsuge et al. 2012. J. Immunol. 188:2524-2536. (Blocking – in vitro)
Shimazu T, Iida R, Zhang Q, Welner RS, Medina KL, Alberola-Ila J, and Kincade PW. 2012. Blood. 119:4889-4897. (Blocking – Flow cytometry)
Stoeker L, Nordone S, Gunderson S, Zhang L, Kajikawa A, LaVoy A, Miller M, Klaenhammer TR, and Dean GA. 2011. Clin. Vaccine Immunol. 18: 1834-1844. (Blocking – Flow cytometry)
Beretta F, St.-Pierre J, Piccirillo CA, and Stevenson MM. 2011. J. Immunol. 186: 4862-4871. (Blocking – Flow cytometry)
Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)
Kurlander RJ, Ellison DM and Hall J. 1984. J Immunol. 133(2): 855-862. (Blocking – in vivo)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.