The UC10-4F10-11 antibody is specific for mouse CD152, commonly known as CTLA-4, a 33-37 kDa protein expressed as a homodimer on the surface of activated T and B cells, and on thymocytes. CTLA-4 is structurally similar, yet functionally disparate, to the T cell co-stimulatory molecule CD28. Both CTLA-4 and CD28 interact with the co-stimulatory molecules CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells, with CTLA-4 displaying a higher avidity than CD28. While CD28 typically delivers a potent co-stimulatory signal in support of T cell activation, CTLA-4 appears to act as a negative regulator of T cell activation and may contribute to the suppressor function of Treg cells.
CTLA-4 proteins may be initially sequestered within Golgi vesicles, from which they can be rapidly transferred to and from the cell surface, a mechanism by which Treg cells can selectively impart suppressive functions. The UC10-4F10-11 antibody may be used for flow cytometric analysis of CTLA-4 expression.
||In Vivo Ready™ Anti-Mouse CD152 (CTLA-4) (UC10-4F10-11)
||Armenian Hamster IgG1, kappa
||In Vivo Ready™
||Flow Cytometry, Functional Assays
||Lischke T, Hegemann A, Gurka S, Van DV, Burmeister Y, Lam K-P, Kershaw O, Mollenkopf H-J, Mages HW, Hutloff A, and Kroczek RA. 2012. J. Immunol. 189: 234-244. (Flow Cytometry).
Tai X, Laethem FV, Pobezinsky L, Guinter T, Sharrow SO, Adams A, Granger L, Kruhlak M, Lindsten T, Thompson CB, Feigenbaum L, and Singer A. 2012. 119: 5155-5163. (Flow Cytometry).
Matheu MP, Su Y, Greenberg ML, Blanc CA, Parker I, Scott DW, and Calahan MD. 2012. 109: E1258-E1266. (in vitro blocking).
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
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