The 2F1 antibody reacts with mouse KLRG1 (Killer cell Lectin-like Receptor G1). This 30-38 kDa homodimeric receptor may be expressed by activated, mature NK cells and by effector/memory T cells, with potentially different roles in each cell type. KLRG1 can regulate, in an inhibitory fashion, the development and effector functions of NK cells, and is often cited as a senescence or terminal differentiation marker for T cells. Ligands for KLRG1 include members of the cadherin family of adhesion molecules, specifically N-Cadherin, E-Cadherin, and R-Cadherin. These interactions may induce bidirectional, immunosuppressive signaling in both KLRG- and Cadherin-expressing cells. A more recently identified role for KLRG1-Cadherin signaling in tissue organization, e.g. in cardiac angiogenesis, expands the function of these interactions beyond immunosuppression of immune cells. (Bouchentouf et al. 2010. J. Immunol. 185: 7014-7025).
The 2F1 antibody may be used as a phenotypic marker for KLRG1 in mouse, frequently in combination with Anti-Mouse CD127 antibody (clone A7R34), for identification of effector T cell populations.
|Name||Purified Anti-Mouse KLRG1 (2F1)|
|Alternative Names||CLEC15A; MAFA; MAFA-2F1; MAFA-L; MAFA-LIKE|
|Isotype||Golden Syrian Hamster IgG|
|Application||Flow Cytometry, IP|
|Citations*||Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).
Tessmer MS, Fugere C, Stevenaert F, Naidenko OV, Chong HJ, Leclercq G, and Brossay L. 2007. Int. Immunol. 19:391-400. (Immunoprecipitation, in vitro blocking, Flow cytometry)
Robbins SH, Nguyen KB, Takahashi N, Mikayama T, Biron CA, and Brossay L. 2002. J. Immunol. 168: 2585-2589. (in vitro blocking)
Hanke T, Corral L, Vance RE, and Raulet DH. 1998. Eur. J. Immunol. 28(12): 4409-4417. (Origination of ≥ 2F1 clone)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.