The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.
The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
|Name||Purified Anti-Mouse CD45 (30-F11)|
|Alternative Names||Leukocyte Common Antigen, LCA, Ly-5|
|Isotype||Rat IgG2b, κ|
|Application||Flow Cytometry, IF, IHCP, IP, Western Blot|
|Citations*||Panchal RG, Ulrich RL, Bradfute SB, Lane D, Ruthel G, Kenny TA, Iversen PL, Anderson AO, Gussio R, Raschke WC, and Bavari S. 2009. 284: 12874-12885. (Immunoprecipitation)
Cherpes TL, Busch JL, Sheridan BS, Harvey SAK, and Hendricks RL. 2008. J. Immunol. 181: 969-975. (Complement-mediated cell depletion)
Cheng G, Zhang H, Yang X, Tzima E, Ewalt KL, Schimmel P, and Faber JE. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1138-R1146. (Immunohistochemistry – paraffin embedded tissue)
Nguyen JT, Evans DP, Galvan M, Pace KE, Leitenberg D, Bui TN, and Baum LG. 2001. J. Immunol. 167: 5697-5707. (Immunofluorescence microscopy, Immunoprecipitation)
Czyzyk J, Leitenberg D, Taylor T, and Bottomly K. 2000. Mol. Cell. Biol. 20(23): 5740-5747. (Western Blot)
Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273: 30680-30687. (Western Blot)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.