The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.
The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.
|Name||Purified Anti-Mouse CD45.1 (A20)|
|Alternative Names||SJL, Ly5.1|
|Isotype||Mouse IgG2a, κ|
|Application||Flow Cytometry, IHCF, IP|
|Citations*||Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109: 8670-8675. (Flow Cytometry)
Siggs OM, Li X, Xia Y, and Beutler B. 2012. J. Exp. Med. 209:19-27. (Flow Cytometry)
Wakim LM, Woodward-Davis A and Bevan MJ. 2010. 107: 17872-17879. (Immunohistochemistry – OCT embedded frozen tissue)
Ato M, Nakano H, Kakiuchi T, and Kaye PM. 2004. J. Immunol. 173: 4815-4820. (Immunohistochemistry – frozen tissue)
Shen F-W, Tung J-S and Boyse EA. 1986. Immunogenetics. 24(3): 146-149. (Immunoprecipitation)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.