The H1.2F3 antibody reacts with mouse CD69, a type II transmembrane glycoprotein also known as the Very Early Activation Antigen, EA-1, Leu23, Activation Inducer Molecule (AIM) and CLEC2C. CD69 is expressed as a 60 kDa disulfide-linked homodimer on activated T and B cells, NK cells, neutrophils and monocytes. Induction occurs rapidly upon activation. It is also constitutively expressed on platelets and a subset of thymocytes. CD69 acts as a co-stimulatory molecule involved in activation and proliferation of T cells, and may be a marker for thymocytes undergoing TCR-mediated positive selection.
Co-stimulation with the H1.2F3 clone has been reported to enhance T cell and macrophage activation. Please choose the appropriate format for each application.
||PerCP-Cyanine5.5 Anti-Mouse CD69 (H1.2F3)
||Very Early Activation Antigen (VEA), AIM, EA-1, Leu23, CLEC2C, MLR3, gp34/28
||Armenian Hamster IgG
||Yamashita I, Nagata T, Tada T and Nakayam T. 1993. Int Immunol. 5(9): 1139-1150.
Ziegler SF, Ramsdell F and Alderson MR. 1994. Stem Cells. 12(5): 456-465.
Marzio R, Jirillo E, Ransijn A, Mauel J and Corradin SB. 1997. J Leukoc Biol. 62(3): 349-355.
Mackay LK, Braun A, Macleaod BL, Collins N, Tebartz C, Bedoui S, Carbone FR and Gebhardt T. 2015. J Immunol. 194(5): 2059-2063. (Flow Cytometry)
Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayam T and Niess JH. 2012. J Immunol. 188(4): 2001-2013. (Flow Cytometry, in vitro activation)
Bremser A, Brack M and Izcue A. 2015. PLoS One. 10(9):e0137393. (Flow Cytometry)
Zhou X, Li F, Kong L, Tomita H, Li C and Cao W. 2005. J Biol Chem. 280(35): 31240-31248. (Immunofluorescence Microscopy)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
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