The RM134L antibody recognizes CD252, also known as OX-40 Ligand or CD134 Ligand, a member of the TNF-α superfamily that is present on the surface of antigen presenting cells and activated B lymphocytes. The OX-40 Ligand interacts with OX-40 (CD134) which is expressed primarily on activated T cells. This costimulatory interaction leads to increased proliferation and IL-2 production responses of activated T cells, and at the same time enhances proliferation and immunoglobulin secretion by activated B cells.
The RM134L antibody is useful for flow cytometric detection of CD252 on stimulated mouse splenocytes. It has also been reported to block the costimulatory activity of ≥≥OX-40 Ligand. Please choose the appropriate format for each application.
||Biotin Anti-Mouse CD252 (OX40 Ligand) (RM134L)
||CD134L, CD134 Ligand, OX-40L, OX-40 Ligand, TNF-αSF4, Tumor necrosis factor ligand superfamily member 4, gp34
||Rat IgG2b, κ
||Akiba H, Oshima H, Takeda K, Atsuta M, Nakano H, Nakajima A, Nohara C, Yagita H and Okumura K. 1999 J Immunol. 162(12): 7058-7066. (Flow cytometry, in vitro blocking)
Stuber E, Neurath M, Calderhead D, Fell HP and Strober W. 1995 Immunity. 2(5): 507-521.
Sibilano R, Frossi B, Suzuki R, D’Incà F, Gri G, Piconese S, Colombo MP, Rivera J and Pucillo CE. 2012 Journal of Allergy Clin Immunol. 130(3): 751–760. (Blocking)
van der Merwe M, Abdelsamed HA, Seth A, Ong T, Vogel P and Pillai AB. 2013 J Immunol. 191(11): 5764-5776 (Flow cytometry)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
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