The XMG1.2 antibody is specific for mouse Interferon-gamma (IFN-g), a 20 kDa type II cytokine known for its central roles in protection against bacterial or viral pathogens and for its anti-tumor properties. IFN-g is secreted by several types of immune cells which allow the cytokine to modulate innate immunity when secreted by NK and NKT cells, and to function in support of adaptive immunity when secreted by Th1 and CD8+ T cells (CTLs).
The XMG1.2 antibody is suitable for detection of intracellular IFN-g protein by flow cytometry. Other formats can be used for quantitative analysis of the secreted protein by ELISA when paired with an appropriate capture antibody. This clone has been reported for neutralization of the functional activity of ≥≥IFN-g in a variety of assays (use format suitable for functional assays).
|Name||APC Anti-Mouse IFN gamma (XMG1.2)|
|Alternative Names||IFN-g, IFNg, Interferon-g, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)|
|Isotype||Rat IgG1, kappa|
|Citations*||Choudhry N, Petry F, van Rooijen N, and McDonald V. 2012. J. of Infect. Disease. 206: 117-124. (in vivo neutralization)
Cobb D and Smeltz RB. 2012. J. Immunol. 188: 3766-3773. (in vitro neutralization)
Brown DM, Lee S, Garcia-Hernandez M, and Swain SL. 2012. J. Virol. 86: 6792-6803. (ELISpot – detection)
Yu H, Karunakaran KP, Jiang X, Shen C, Andersen P, and Brunham RC. 2012. Infect. Immun. 80: 1510-1518. (ELISpot -detection)
Kwon M-J, Ma J, Ding Y, Wang R, and Sun Z. 2012. J. Immunol. 188: 5887-5897. (in vitro induction of Th2 polarization)
Barr TA, Shen P, Brown S, Lampropoulou V, Roch T, Lawrie S, Fan B, O’Connor RA, Anderton SM, Bar-Or Am Fillatreau S, and Gray D. 2012. J. Exp. Med. 209: 1001-1010. (Flow cytometry)
Cardona AE, Restrepo BI, Jaramillo JM, and Teale JM. 1999. J. Immunol. 162: 995-1002. (Immunohistochemistry – frozen tissue)
Kupfer A, Mosmann TR, and Kupfer H. 1991. Proc. Natl. Acad. Sci. 88: 775-779. (Immunofluorescence microscopy)
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.