7-AAD (7-Aminoactinomycin D) is a nucleic acid dye that can be used to exclude nonviable cells from the analysis of flow cytometry data.
Kamachi F, Isshiki T, Harada N, Akiba H and Miyake S. 2015. Biochem Biophys Res Commun. doi: 10.1016/j.bbrc.2015.06.005. (Flow Cytometry)
||Propidium Iodide or 7-AAD Viability Staining Protocol
||Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
||10 mM NaH2PO4, 150 mM Na10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2Cl, 0.09% NaN3, pH 7.2
Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
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