Tonbo Biosciences Foxp3 / Transcription Factor Staining Buffer Kit contains specially formulated buffers and solutions for optimal resolution and low background in your analysis of nuclear antigens by flow cytometry. This complete kit contains the following components for use in these staining protocols for detection of nuclear antigens such as Foxp3 and ROR gamma.
Foxp3 / Transcription Factor Fix/Perm Concentrate (4X) (Cat. No. TNB-1020-L050): 50 mL. A concentrated solution which, when diluted with Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (cat. no. TNB-1022-L160), provides best results in protocols for intranuclear staining of transcription factors using fluorescently conjugated antibodies.
Foxp3 / Transcription Factor Fix/Perm Diluent (1X) (Cat. No. TNB-1022-L160): 160 mL. Intended for use as a diluent for Foxp3 / Transcription Factor Fix/Perm Concentrate (4X)
Flow Cytometry Perm Buffer (10X) (Cat. No. TNB-1213-L150): 150 mL. Provided as a concentrate which, when diluted with distilled water to a 1X solution, provides best results in intracellular staining protocols for cytokines and other cytoplasmic antigens, by maintaining membrane permeabilization throughout staining and wash steps.
|Name||Foxp3 / Transcription Factor Staining Buffer Kit|
Application Key :
FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy
*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.
30105 T- CellBrite™ Steady 405, 30105- CellBrite™ Steady 405, 30106 – CellBrite™ Steady 488, 30106 T – CellBrite™ Steady 488, 30107 – CellBrite™ Steady 550, 30107 T – CellBrite™ Steady 550, 30108 – CellBrite™ Steady 650, 30108 T – CellBrite™ Steady 650, 30109 – CellBrite™ Steady 685, 30109 T – CellBrite™ Steady 685
100 labelings, 500 labelings