Product Name: Tumor-derived Exosome enrichment and Quantification, Assay Kit
Full Product Name: ExoQuant Tumor-derived Exosome enrichment and Quantification Assay Kit (Biological Fluids/Cell Media; Colorimetric)
Sample Manual Insert: Download Sample PDF Manual View Sample PDF Manual
Assay Type: Quantitative Sandwich
Related Product Information for
Tumor-derived Exosome enrichment and Quantification assay kit
Background/Introduction: Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. ExoQuant Kit is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. ExoQuantTM consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. ExoQuantTM Tumor-derived Exosome enrichment and Quantification Assay Kit includes an immunoplate able to enrich the tumor exosome subpopulation and is especially suitable for biomarker discovery in clinical cancer research. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).
Product Categories/Family for Tumor-derived Exosome enrichment and Quantification assay kit
Application Notes for Tumor-derived Exosome enrichment and Quantification assay kit
Tumor-derived exosome enrichment and quantification from Biological fluids
Sample Manual Insert of MBS846491.
https://cdn.mybiosource.com/tds/protocol_manuals/800000-9999999/MBS846491.pdf
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Unit/Price | 100 Assays / $990 +1 FREE 8GB USB, 5×100 Assays / $4,590 +5 FREE 8GB USB |
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