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Donkey Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

52.00 USD128.00 USD

Conjugation

volume

Cat. No

Clear

Description

This is a highly cross-adsorbed donkey anti-rabbit IgG (H+L) secondary antibody that has a low degree of labeling (DOL) with one of our bright and photostable CF® dyes.

  • CF® dye single label secondary antibody ideal for STORM imaging
  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 7 bright and photostable CF® dyes

Secondary antibodies with a low DOL, or number of dye molecules per antibody molecule, have been reported to be optimal for STORM (Bittel et al. (2015) Proc. SPIE 9331). This product is prepared by single labeling (DOL=1) of highly cross-adsorbed goat anti-mouse IgG (H+L) with a selection of compatible CF® dyes for (d)-STORM super-resolution microscopy. To minimize cross-reactivity, the antibody has been adsorbed against human, bovine, horse, rabbit, and swine serum.

Learn more about CF® Dyes for super-resolution microscopy.

CF® Dye Secondary Antibodies, Single Label for STORM

Dye (Ex/Em) Donkey Anti-Goat Donkey Anti-Guinea Pig Donkey Anti-Mouse Donkey Anti-Rabbit Goat Anti-Mouse Goat Anti-Rabbit
Cross-adsorption Ck, GP, Hs, Hu, Ms, Rb, Rt, SHm Bv, Ck, Gt, Hs, Hu, Ms, Rb, Rt, Shp, SHm Bv, Ck, Gt, GP, Hs, Hu, Rb, Shp, SHm Bv, Ck, Gt, GP, Hs, Hu, Ms, Rt, Shp, SHm Bv, Hs, Hu, Rb, Sw Hu, Ms, Rt
CF®535ST
(535/568 nm)
20823 20824 20821 20822
CF®568
(562/583 nm)
20836 20838 20802 20803 20800 20801
CF®594ST
(593/614 nm)
20806 20807 20804 20805
CF®647
(650/665 nm)
20829 20837 20806 20811 20808 20809
CF®660C
(667/685 nm)
20815 20816 20812 20813
CF®680
(681/698 nm)
20819 20820 20817 20818
CF®750
(755/777 nm)
20827 20828 20825 20826

PRODUCT ATTRIBUTES

Clonality Polyclonal
Host species Donkey
Reactivity (target) Rabbit IgG
Species reactivity Rabbit
Cross adsorption Bovine, Chicken, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian hamster
Antibody type Secondary
Concentration 1 mg/mL
Applications STORM

Bv: bovine; Ch: chicken; Gt: goat; GP: guinea pig; Hs: horse; Hu: human; Ms: mouse; Rb: rabbit; Sh: sheep; SHm: Syrian hamster; Sw: swine; Rt: rat

CF® dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

CF is a registered trademark of Biotium, Inc.

REFERENCES

Download a list of CF® dye references.

  1. Proc. SPIE 9331, (2015), DOI: 10.1117/12.2083209

PROTOCOLS

SDS

SDS CF dye antibody conjugate

SUPPORTING DOCUMENTS

Additional information

Conjugation

CF535ST, CF568, CF594ST, CF647, CF660C, CF680, CF750

volume

50uL, 500uL

Cat. No

20803-500uL, 20803-50uL, 20807-500uL, 20807-50uL, 20811-500uL, 20811-50uL, 20816-500uL, 20816-50uL, 20820-500uL, 20820-50uL, 20824-500uL, 20824-50uL, 20828-500uL, 20828-50uL

Conjugation

volume

Cat. No

Clear