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Permeabilization and Blocking Buffer (5X)

143.00 USD

Description

A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

Product Description

The Permeabilization and Blocking Buffer (5X) is a concentrated stock solution for one step cell permeabilization and blocking. It is designed for use during immunofluorescence staining for fluorescence microscopy.

  • Permeabilizes fixed cells for immunofluorescence staining
  • Blocks non-specific binding of antibodies
  • Can be used to dilute antibodies for staining, and as a wash buffer
  • Contains goat serum, not for use with anti-goat or anti-sheep antibodies
  • 100 mL of 5X solution

Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer (22016) and 10% Fish Gelatin Blocking Agent (22010) for use with anti-goat and anti-sheep secondary antibodies.

Product Attributes

Storage Conditions Store at -10 to -35 °C

Protocols

SDS

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