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µ-Slide VI 0.1

231.00 USD386.00 USD

Surface Modification

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Description

Product with ibidi Polymer Coverslip #1.5 bottom Optimized for inverted microscopy Suitable for adherent cells Suitable for immunofluorescence assays Suitable for cell culture under flow Optimized for phase contrast microscopy

 

A six channel µ-Slide for parallel flow assays and immunofluorescence that uses a minimal amount of reagents and medium

  • Perfect for rolling and adhesion assays on cell monolayers or protein coatings
  • Minimal amounts of cells and/or liquids needed
  • Medium-to-high shear stress that is applicable with small volumes

80666

Applications

  • Flow assays that use a minimum of cells and/or liquids (mouse model)
  • Parallel shear stress applications
  • Immunofluorescence microscopy after perfusion
  • High-resolution microscopy of cells under shear stress


NOTE:
Not for use in static cultures

Specifications

Outer dimensions (w x l) 75.5 x 25.5 mm²
Adapters Female Luer
Number of channels 6
Channel volume 1.7 µl
Channel height 0.1 mm
Channel length 17 mm
Channel width 1 mm
Volume per reservoir 60 µl
Growth area 0.17 cm2 per channel
Coating area using 1.7 µl 0.34 cm² per channel
Bottom: ibidi Polymer Coverslip
Define and print your
experimental setup

Technical Features

  • Six parallel channels on one slide
  • Small sample amounts
  • A low dead volume
  • Smallest ibidi channel slide with highest shear stress
  • Easy connection to tubes and pumps using a female Luer adapter
  • Defined shear stress and shear rate levels

Experimental Example

HUVEC, cultivated over 7 days at 10 dyn / cm². VE-cadherins are stained in green, cell nuclei are stained in blue.

HUVEC in the µ-Slide VI 0.1 channel, cultivated over 1 day at 10 dyn / cm².

Adhesion Assay

Additional information

Surface Modification

Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized, Collagen IV: #1.5 polymer coverslip, sterilized

Pcs./Box

15

Supporting Material

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