To test for your application, choose the Trial Pack with 2 slides in the “Pcs./Box” field below.
Free samples are not available for this product.
in cooperation with
Salvermoser, Melanie, et al. “Myosin 1f is specifically required for neutrophil migration in 3D environments during acute inflammation.” Blood, The Journal of the American Society of Hematology 131.17 (2018): 1887-1898. 10.1182/blood-2017-10-811851
Rohwedder, Ina, et al. “Src family kinase-mediated vesicle trafficking is critical for neutrophil basement membrane penetration.” Haematologica (2019). 10.3324/haematol.2019.225722
Shown here is a time lapse movie of a transmigrating human HL-60 cell that was seeded into the lower channel of a µ-Slide Membrane ibiPore Flow (pore size 3 µm). Under flow conditions, the cell transmigrates in vitro through the porous glass membrane into the upper channel that was filled with a 3D collagen matrix containing the chemoattractant fMLP.
Live cell imaging of a transmigrating single SiR-Actin-stained cell (indicated by pseudo colors) by spinning disc confocal microscopy (20x objective) with a time interval of 30 s. The arrow indicates the direction of flow at a shear stress of 1 dyne/cm2; Scale bar = 10 um. Courtesy R. Pick, Munich, Germany.
|Total coating area||4.50 cm²|
|Bottom||ibidi Polymer Coverslip|
|Main Channel (Lower Channel)|
|Access||Luer port, accessible with female Luers|
|Growth area||1.25 cm²|
|Access||Reservoir port, accessible with 20/200 µl pipet tips|
|Height over membrane||1.3 mm|
|Thickness||0.3 µm (300 nm)|
|Membrane size||2 mm x 2 mm|
|Porous area||1.77 mm x 1.84 mm|
|Restrictions for objective lenses||Working distance >0.5 mm|
|Pore layout||Hexagonal spacing|
The μ-Slide Membrane ibiPore Flow consists of a horizontal porous glass membrane that is inserted between two channels. The upper channel is a static reservoir above the membrane. The lower channel is a perfusion channel for applying defined shear stress on cells, which are attached to the membrane. The upper and the lower channel communicate with each other only across the membrane.
Porosity refers to the void volume fraction of the membrane. It is defined as the volume of the pores divided by the total volume of the membrane.
|Membrane Pore Size and Porosity||Applications||Example Cell Types|
|0.5 µm pores,
high porosity (20%)
|Permeability and transport studies,
|Lung cells, epithelial cells|
|3.0 µm pores,
low porosity (5%)
|Transendothelial migration||Leukocytes (e.g., neutrophils), lymphocytes (e.g., T cells or B cells)|
|5.0 µm pores,
low porosity (5%)
|Invasion, migration||Monocytes, macrophages, lymphocytes (e.g., T cells or B cells)|
|8.0 µm pores,
low porosity (5%)
|Invasion, migration||Tumor and cancer cells, endothelial and epithelial cells, fibroblasts, osteoblasts, melanoma cells, glioma cells|
The bigger the cell, the bigger the recommended pore size.
Human endothelial cells on 3 µm ibiPore membrane after coating with Collagen Type IV according to Application Note 08 (PDF). Immunofluorescence overlay image of phase contrast, DAPI (blue), VE-cadherin (green), F-actin (red).
Fibroblasts on 3 µm ibiPore membrane. Phase contrast image, objective lens 4x.
Cells on upper side of the membrane
Cells on lower side of the membrane
Phase contrast microscopy of A549 lung cells (adenocarcinomic human alveolar basal epithelial cells) cultured on the µ-Slide Membrane ibiPore Flow, pore size 3 µm, 5% porosity. Left: cells were seeded on the upper side of the membrane. Right: cells were seeded on the lower side of the membrane. Data by Dr. Anita Reiser, Chair of Prof. Rädler, Ludwig-Maximilians-University Munich.
These applications have been tested by the ibidi R&D team or by our customers.
A cell monolayer is cultivated on one side of the membrane.
Cells on the lower side of the membrane
Cells on the lower side of the membrane with defined shear stress
Cells on the upper side of the membrane
Two separate cell monolayers are cultivated on each side of the membrane. With this method, signaling, co-culture, and transport studies are possible.
Co-culture of two different cell types on both sides of the membrane
Chemical factors inside of a 3D gel matrix lead to the polarization of a cell monolayer that is cultured on the other side of the membrane.
Cells on the lower side of the membrane with a 3D gel matrix in the upper channel
Cells on the lower side of the membrane with a 3D gel matrix with embedded cells in the upper channel
The following examples illustrate further potential product uses. ibidi has not yet tested these applications in-house, therefore we cannot provide specific protocols. However, from a technical point of view, these applications should be possible.
We would appreciate your feedback for any application of the µ-Slide Membrane ibiPore Flow that works for you. Please send your images or videos with a short description to firstname.lastname@example.org and we will be happy to publish them on our website to support the scientific community (e.g., as User Protocol).
A cell monolayer is cultivated on one side of the membrane to observe cellular trans-membrane migration.
Transmigration of suspended leukocytes
Rolling, adhesion, and transmigration of leukocytes, for example, under defined shear stress through a monolayer of cells on the lower side of the membrane
A polarized cell monolayer (e.g., lung epithelial cells or skin cells) is seeded on one side of the membrane, which is exposed to air.
Epithelial cells, such as lung cells, on the upper side of the membrane, exposed to air in the upper channel and supplied by culture medium from the lower channel
Under flow conditions, the rolling, adhesion, and transmigration of leukocytes towards chemoattractant-producing cancer cells in a 3D matrix can be observed.
Transmigration of cells across a cell monolayer on the membrane into a 3D gel matrix with embedded cells
Due to technical reasons, the following applications will not work with this product and should be avoided.
This product is not intended for:
3 µm pores
3 µm single pore
0.5 µm pores
0.5 µm single pores
ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized
10, 2 (Trial Pack), 10, Kit incl. (petri dishes, syringes, pipet tips), 2 (Trial Pack), Kit incl. (petri dishes, syringes, pipet tips)
3.0 µm pore size; 5% porosity, 5.0 µm pore size; 5% porosity, 8.0 µm pore size; 5% porosity, 0.5 µm pore size; 20% porosity