|Outer dimensions (w x l)||25.5 x 75.5 mm²|
|Number of wells||3|
|Volume of wells||16 μl|
|Well dimensions||5.4 mm x 4.0 mm|
|Well height (without channel)||0.8 mm|
|Growth area per well||0.21 cm²|
|Coating area per well||0.34 cm²|
|Channel width||5.0 mm|
|Channel volume (without wells)||150 μl|
|Channel height (without well)||0.6 mm|
|Volume per reservoir||60 μl|
|Top cover:||ibidi Polymer Coverslip|
|Bottom:||ibidi Polymer Coverslip|
The Principle of the µ-Slide I Luer 3D:
The μ-Slide I Luer 3D has three wells with one channel on top for culturing cells on a 3D gel matrix with defined flow. Each well can be
filled with a gel, on which cells can be cultivated and microscopically investigated. The channel can be connected to a pump (e.g., to the ibidi Pump System) for the application ofdefined shear stress.
Using this method, an in vivo-like functional monolayer (e.g., an endothelial barrier) can be established on the gel matrix without any artificial filters or membranes involved. The μ-Slide I Luer 3D is especially designed for the simulation of blood vessels using endothelial cells.
Standard Filling Procedure:
The µ-Slide I Luer 3D is a versatile channel slide for a huge variety of cell culture applications using a 3D gel matrix and shear stress:
These applications have been tested by the ibidi R&D team or by our customers.
Endothelial Barriers Without Artificial Membrane:
The µ-Slide I Luer 3D allows for creating an endothelial barrier without the need of an artificial filter membrane. Endothelial cells can be seeded on a suitable gel matrix, such as Collagen Type I, Rat Tail. After connecting the slide to a pump and applying defined shear stress, an in vivo-like endothelial barrier assay is created. This model simulates blood vessels on a soft ECM matrix instead of using a hard filter membrane.
A cell monolayer is seeded on a hydrogel and defined shear stress can be applied. Being compatible with all pipettable gel matrices, the µ-Slide I Luer 3D allows a wide variety of polarization studies (e.g., with epithelial cells).
The following examples illustrate further potential product uses. ibidi has not yet tested these applications in-house, therefore we cannot provide specific protocols. However, from a technical point of view, these applications should be possible.
3D Cell and Tissue Assays:
Several three-dimensional cell culture experiments can be performed using the µ-Slide I Luer 3D. For example, single cells or cell clusters can be seeded in the hydrogel matrix. Optionally, defined shear stress can be applied to a cell monolayer on top.
Rolling, Adhesion, and Transmigration Assays:
For physiological rolling, adhesion, and transmigration approaches, the endothelial cell layer can be supplied with suspension cells on the luminal side and target cancer cells on the basal side. Optionally, defined shear stress can be applied.
Decision Guide: ibidi Solutions for Two-Compartment and Membrane Assays:
µ-Slide I Luer 3D
|Principle of interface||Gel-based||Membrane-based|
|Substrate for cells||Soft hydrogels (e.g., collagen or Matrigel)||Hard glass membrane with different pore sizes|
|Microscopy||Restricted by gel’s optics||No restriction|
|Flow application||Defined shear stress in the channel||Defined shear stress in the lower channel|
|Air-liquid interphase (ALI)||No||Yes|
ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized, Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized