The sprouting potential of a cell cluster can be conveniently analyzed using the µ-Slide Angiogenesis. To carry out a sprouting assay, spheroids or pieces of tissue (e.g., from the aorta), are placed onto or directly in the gel matrix before imaging.
3D Cell Culture
In many cases, a 3D environment more closely resembles an in vivo situation than a 2D cell culture. The µ-Slide Angiogenesis is an easy and cost-effective solution for the cultivation and microscopy of cells embedded in gel matrices. The gel layer is directly connected to the medium reservoir above, which allows for fast and easy medium exchange by diffusion. Also, non-adherent cells, such as blood cells or bacteria, can be immobilized for enhanced microscopy access.
For analyzing the outcome of a tube formation or a 3D culture experiment, immunofluorescence staining can be performed in the µ-Slide Angiogenesis. The cells can be fixated and stained directly on or in the gel, then imaged using high-resolution microscopy.
When using less than 10 µl of gel to fill the inner well of the µ-Slide Angiogenesis, small numbers of cells can be cultivated and focused on a soft gel surface.
Low Volume Microscopy Chamber
The µ-Slide Angiogenesis can also be used as a low-volume microscopy chamber without any gel, which is useful for experiments where several wells with a low volume are required.