240.00 USD
key features
| Outer dimensions in mm (w x l) | 25.5 x 75.5 |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) in mm | 9.4 x 10.7 x 6.8 |
| Volume per well | 300 µl |
| Total height with lid | 8 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well | 2.20 cm² |
| Bottom: ibidi Polymer Coverslip | |
Define and print your experimental setup
The ibidi µ-Slide 8 well allows standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (i.e., cell cultivation, fixation, staining and imaging) are carried out in the classic open well geometry. After staining, the sample can be observed through the coverslip-like bottom using high resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.
Closely fitting lid
Suitable for high resolution microscopy
To test different compound concentrations, e.g., in stem cell development
The µ-Slide 8 Well enables high-resolution live cell imaging using different brightfield and fluorescence techniques. Here, live cell microscopy was performed using the µ-Slide 8 Well in the ibidi Heating System, Universal Fit, for 1 Chamber on a Nikon Eclipse TIE inverted microscope.
Live cell imaging in a µ-Slide 8 Well using transmitted light.
Live cell imaging in a µ-Slide 8 Well using fluorescence.
F-actin Visualization in Living Cells Using LifeAct-TagGFP2 Protein
Live cell imaging of F-actin in Rat1 fibroblasts after LifeAct-TagGFP2 Protein transfer (30 µg/ml, 3 minutes) using the µ-Slide 8 Well.
Live Cell Imaging of Algae
Brightfield microscopy of the green freshwater algae Haematococcus pluvialis in a µ-Slide 8 Well. The cells are in an immature cyst state with developing centers, filled with the strong antioxidant astaxanthin (red), and surrounded by Chlorophyll from the chloroplasts (green). 40x objective lens.
Live Cell Imaging of Fibroblasts After Transfection
Live cell imaging on an inverted widefield fluorescence microscope. Rat fibroblast cells 24 hours after transfection with pCMV-eGFP. Red: Rhodamine-labelled fluorescent lipoplexes, Green: eGFP fluorescence. 60x objective lens.
DIC Microscopy of Mammalian Cells
Differential interference contrast (DIC) microscopy of mammalian cell culture (fibroblasts) using the µ-Slide 8 Well and the DIC lid for µ-Slides. 63x objective lens.
