Mini Cart

µ-Slide 8 Well

240.00 USD


An open µ-Slide (chambered coverslip) with 8 wells for use in immunofluorescence and high-end microscopy

key features

  • The all-in-one chamber slide reduces the number of experimental steps in immunofluorescence protocols
  • Observation of the sample through the coverslip-like bottom using high resolution microscopy
  • Fast and easy sample preparation: No coverslips, no leakage
  • Cost-effective experiments using small numbers of cells and low volumes of reagents

Instruction menu

Instruction menu
  • Cultivation and microscopy of cell cultures
  • Transfection assays
  • Immunofluorescence staining
  • Fluorescence microscopy of living and fixed cells
  • Live cell imaging over extended time periods
  • Suitable for DIC, when used with a DIC lid
Outer dimensions in mm (w x l) 25.5 x 75.5
Number of wells 8
Dimensions of wells (w x l x h) in mm 9.4 x 10.7 x 6.8
Volume per well 300 µl
Total height with lid 8 mm
Growth area per well 1.0 cm²
Coating area per well 2.20 cm²
Bottom: ibidi Polymer Coverslip

Define and print your experimental setup

Technical Features
  • Open µ-Slide (chambered coverslip) with 8 independent wells
  • Excellent optical quality imaging chamber slide for high-end microscopy
  • Compatible with staining and fixation
  • ibiTreat surface for optimal cell adhesion
  • Biocompatible plastic material – no glue, no leaking
  • Also available in an adhesive version without a bottom: sticky-Slide 8 Well
  • Also available with a glass bottom or with a grid

The ibidi µ-Slide 8 well allows standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (i.e., cell cultivation, fixation, staining and imaging) are carried out in the classic open well geometry. After staining, the sample can be observed through the coverslip-like bottom using high resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.

Closely fitting lid
Suitable for high resolution microscopy

Other Possible Applications

To test different compound concentrations, e.g., in stem cell development


Live Cell Imaging

The µ-Slide 8 Well enables high-resolution live cell imaging using different brightfield and fluorescence techniques. Here, live cell microscopy was performed using the µ-Slide 8 Well in the ibidi Heating System, Universal Fit, for 1 Chamber on a Nikon Eclipse TIE inverted microscope.

Live cell imaging in a µ-Slide 8 Well using transmitted light.

Live cell imaging in a µ-Slide 8 Well using fluorescence.

Live Cell Imaging Examples

F-actin Visualization in Living Cells Using LifeAct-TagGFP2 Protein
Live cell imaging of F-actin in Rat1 fibroblasts after LifeAct-TagGFP2 Protein transfer (30 µg/ml, 3 minutes) using the µ-Slide 8 Well.

Live Cell Imaging of Algae
Brightfield microscopy of the green freshwater algae Haematococcus pluvialis in a µ-Slide 8 Well. The cells are in an immature cyst state with developing centers, filled with the strong antioxidant astaxanthin (red), and surrounded by Chlorophyll from the chloroplasts (green). 40x objective lens.

Live Cell Imaging of Fibroblasts After Transfection
Live cell imaging on an inverted widefield fluorescence microscope. Rat fibroblast cells 24 hours after transfection with pCMV-eGFP. Red: Rhodamine-labelled fluorescent lipoplexes, Green: eGFP fluorescence. 60x objective lens.


DIC Microscopy of Mammalian Cells
Differential interference contrast (DIC) microscopy of mammalian cell culture (fibroblasts) using the µ-Slide 8 Well and the DIC lid for µ-Slides. 63x objective lens.