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µ-Slide 8 Well high

210.00 USD975.00 USD

Surface Modification

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Description

Product description:

 

A chambered coverslip with 8 individual wells and high walls for cell culture, immunofluorescence, and high-end microscopy
  • All-in-one 8 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
  • High-resolution imaging through No. 1.5 polymer coverslip bottom with the highest optical quality
  • Suitable for various microscopy techniques (e.g., DICwidefield fluorescenceconfocal microscopytwo-photon microscopyFRAPFRETFLIM, and LSFM)
  • Extra high individual walls to keep cross contamination between wells as low as possible

 

Applications:

 

Technical Features:

      • Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
      • ibiTreat (tissue culture-treated) surface for optimal cell adhesion
      • Imaging chamber slide with excellent optical quality for high-end microscopy
      • Individual well walls for minimizing well-to-well crosstalk and contaminations
      • Compatible with staining and fixation solutions
      • Biocompatible plastic material—no glue, no leaking
      • Also available as an adhesive version without a bottom: sticky-Slide 8 Well high
      • Also available with a Glass Coverslip Bottom: µ-Slide 8 Well high Glass Bottom  for special microscopic applications
      • Additional version available with a 500 µm grid: µ-Slide 8 Well high Grid-500

 

Specifications: 

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 8
Dimensions of wells (w x l x h) 9.4 x 10.7 x 9.3 mm³
Volume per well 300 µl
Height with/without lid 10.8/9.5 mm
Growth area per well 1.0 cm²
Coating area per well2.2  cm²
Bottom: ibidi Polymer Coverslip

Technical Drawing

Technical drawings and details are available in the Instructions (PDF).

 

The Principle of the µ-Slide 8 Well high

 

The Coverslip Bottom

The µ-Slide 8 Well high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 8 Well high without any bottom and the µ-Slide 8 Well high Glass Bottom for special microscopic applications.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.

 

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Find more information about the different surfaces of the ibidi chambers here.

 

 

Fluorescence microscopy of HuH7 cells after mRNA transfection with eGFP (green). Actin cytoskeleton (Phalloidin, yellow), nuclei (DAPI, blue), mRNA (FISH,Quasar 670, red), 40x objective lens, oil immersion.
Application Examples:

Immunofluorescence

The ibidi µ-Slide 8 Well high allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.

S_8082X_8well_workflow.jpg

 

 

 

 

 

 

 

Live Cell Imaging

Timelapse phase contrast microscopy of rat fibroblast cells using the µ-Slide 8 Well high ibiTreat in combination with the ibidi Heating System and the ibidi Gas Incubation System. 63x objective lens, oil immersion, 3 min image intervals for 23 hours.

The µ-Slide 8 Well high enables high-resolution live cell imaging using different microscopy techniques.

Together with the ibidi 

Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.

 

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

 

 

 

 

Instructions

 

Application Guides

 

Application Notes

 

Product Flyer

 

FAQ

Additional information

Surface Modification

Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized, Collagen IV: #1.5 polymer coverslip, sterilized, Poly-L-Lysine: #1.5 polymer coverslip, sterilized, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized

Pcs./Box

15, 90

Surface Modification

Pcs./Box

Clear