Find more information and technical details about the coverslip bottom of the ibidi chambers here.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
Find more information about the different surfaces of the ibidi chambers here.
The ibidi µ-Slide 8 Well allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.
The µ-Slide 8 Well enables high-resolution live cell imaging using different brightfield and fluorescence techniques. Here, live cell microscopy was performed using the µ-Slide 8 Well in the ibidi Heating System, Universal Fit, for 1 Chamber on a Nikon Eclipse TIE inverted microscope.
Live cell imaging in a µ-Slide 8 Well using transmitted light.
Live cell imaging in a µ-Slide 8 Well using fluorescence.
F-actin Visualization in Living Cells Using LifeAct-TagGFP2 Protein
Live cell imaging of F-actin in Rat1 fibroblasts after LifeAct-TagGFP2 Protein transfer (30 µg/ml, 3 minutes) using the µ-Slide 8 Well.
Live Cell Imaging of Algae
Brightfield microscopy of the green freshwater algae Haematococcus pluvialis in a µ-Slide 8 Well. The cells are in an immature cyst state with developing centers, filled with the strong antioxidant astaxanthin (red), and surrounded by Chlorophyll from the chloroplasts (green). 40x objective lens.
Live Cell Imaging of Fibroblasts After Transfection
Live cell imaging on an inverted widefield fluorescence microscope. Rat fibroblast cells 24 hours after transfection with pCMV-eGFP. Red: Rhodamine-labelled fluorescent lipoplexes, Green: eGFP fluorescence. 60x objective lens.
Wenting You, University of Maastricht, The Netherlands
“I tested various slides and found that the µ-Slide 8 Well worked very well. I use the slides for confocal microscopy imaging. My cells are very easy to detach from glass bottom cell culture slides. Therefore, I tried the slides with a Polymer Coverslip bottom. The cells adhere to the cell culture slide very well, and I can get high-resolution images. I have already ordered more polymer cell culture slides from ibidi.”
Johanna Hol Fosse, Norwegian Veterinary Institute, Oslo, Norway
I have tested the µ-Slides 8 Well on adherent fish cell lines, originally isolated from salmon head kidney (ASK and SHK cells) and have been very happy with them. The ASK cells preferred the ibiTreat slides, while the SHK cells grew well on both ibiTreat and Collagen IV. I also tested the ASK cells in a µ-Slide 18 Well – Flat and that worked well, provided that the medium (L-15, 10% FCS) was changed at least every other day. Cells were seeded 5,000/well in the µ-Slide 18 Well – Flat and 50,000/well in the µ-Slide 8 Well. I will place an order within the next few weeks.”
Yuki Manabe, Kyoto University, Graduate School of Agriculture, Kyoto, Japan
“Thank you for your great product µ-Slide 8 Well. I think it is the best for obtaining fluorescent images of adherent cells such as RAW264 and RBL-2H3 using oil immersion microscopy. Thanks to this slide, we can get various good results.”
Yuki Manabe Kyoto University Graduate School of Agriculture Kyoto Japan
Michelle Somerville, Cardiff University, Cardiff, United Kingdom
“We have tested the µ-Slide Angiogenesis and the µ-Slide 8 Well and have found them to be great products. The slides are being used for imaging fluorescently labelled organoids using confocal microscopy and we will continue to use them. I have already recommended them to a lab colleague and will continue to recommend them to others.”
Michelle Somerville Cardiff University Cardiff United Kingdom
Lee Shu Ying, National University of Singapore, Singapore
“As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.
We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling.”
Lee Shu Ying
National University of Singapore
Yong Loo Lin School of Medicine
Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA
“The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”
Brigham Regenerative Medicine Center
“I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”
Stefanie Kirchberger, Children’s Cancer Research Institute, Vienna, Austria
“We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!”
Children’s Cancer Research Institute
Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom
“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“
University of Cambridge
Owen McCarty, PhD, Oregon Health & Science University, Portland, USA
“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”
Owen McCarty, PhD
Department of Biomedical Engineering
Oregon Health & Science University
“I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are ‘slide’ sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great.”
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
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