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Description
Product description:
A chambered coverslip with 18 wells for cell culture, immunofluorescence, and high-end microscopy
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- All-in-one 18 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
- A microscopy slide that is ideal for high-resolution imaging through the No. 1.5 polymer coverslip bottom with the highest optical quality
- A cell culture chamber suitable for various microscopy techniques (g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)
Applications:
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- Cultivation and high-resolution microscopy of cells
- Immunofluorescence staining and fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) microscopy when used with a DIC lid
Technical Features:
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- Chambered coverslip with 18 independent wells and a non-removable polymer coverslip-bottom
- ibiTreat (tissue culture-treated) surface for optimal cell adhesion
- Imaging chamber slide with excellent optical quality for high-end microscopy
- Closely fitting lid for low evaporation
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
- Biocompatible plastic material—no glue, no leaking
- Also available as an adhesive version without a bottom: sticky-Slide 18 Well
- Also available with a Glass Coverslip Bottom: µ-Slide 18 Well Glass Bottom for special microscopic applications
- Chambered coverslip with 18 independent wells and a non-removable polymer coverslip-bottom
Specifications:
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 18 |
| Dimensions of wells (w x l x h) | 5.7 x 6.1 x 6.8 mm³ |
| Volume per well | 100 µl |
| Height with/without lid | 8.2/6.8 mm |
| Growth area per well | 0.34 cm² |
| Coating area per well | 1.15 cm² |
| Bottom: ibidi Polymer Coverslip | |
The Principle of the µ-Slide 18 Well
The Coverslip Bottom
The µ-Slide 18 Well comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 18 Well without any bottom, or and as the µ-Slide 18 Well Glass Bottom for special microscopic applications.
Find more information and technical details about the coverslip bottom of the ibidi chambers here.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
Find more information about the different surfaces of the ibidi chambers here.
Application Examples:
Multiple conditions on one µ-Slide: For immunofluorescence stainings, toxicological screenings, cell surface coatings.
Protocol:
Application note:
Immunofluorescence Staining Using the µ-Slide 18 Well (pdf)
FAQs:
Additional information
| Cat. No | 81811 – Uncoated: #1.5 polymer coverslip , hydrophobic , sterilized, 81816 – ibiTreat: #1.5 polymer coverslip, tissue culture treated , sterilized |
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