65.00 USD – 437.00 USD
NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.
NucSpot® Live 488 stains nuclei with green fluorescence for the FITC channel, while NucSpot® Live 650 has far-red fluorescence for the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 4). The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of the efflux pump inhibitor verapamil for optional use, which may increase probe retention and live cell staining in some cell types. Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.
NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Cy Dye is a registered trademark of GE Healthcare.
|Dye||NucSpot® Live 488, NucSpot® Live 650|
|For live or fixed cells||For fixed cells, For live/intact cells|
|Assay type/options||Long term staining (24-72h), No-wash staining, Real-time imaging|
|Cell permeability||Membrane permeant|
|Fixation options||Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)|
NucSpot® Live 488, NucSpot® Live 650
Trial size, 1 kit
40081, 40081-T, 40082, 40082-T
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