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LysoView™ Dyes

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Description

LysoView™ fluorescent dyes label lysosomes in live cells without a wash step, and are available in multiple colors with options for super-resolution imaging.

Product Description

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

  • No-wash, fluorescent staining of lysosomes in live cells
  • Non-toxic for real-time live-cell imaging
  • Lower background than LyosoTracker® dyes
  • Dye options for super-resolution imaging
  • Blue, green, red, or far-red fluorescence

LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in the low pH environment of the lysosomes, resulting in highly specific lysosomal staining without the need for a wash step. LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.

LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.

LysoView™ Dyes

Product Abs/Em Detection channel Catalog number Size
LysoView™ 405,
1000X in DMSO
318, 400/464 nm DAPI 70066-T 10 uL
70066 50 uL
LysoView™ 488,
1000X in DMSO
506/532 nm GFP, FITC 70067-T 10 uL
70067 50 uL
LysoView™ 540,
1000X in DMSO
541/634 nm (pH ≤5) Rhodamine, Cy®3 70061-T 10 uL
70061 50 uL
LysoView™ 633
(lyophilized solid)
634/659 nm (pH ≤5) Cy®5 70058-T 1 vial*
70058 10 vials*
LysoView™ 650,
1000X in DMSO
650/675 nm Cy®5 70059-T 10 uL
70059 50 uL

*Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution. Cy3 and Cy5 are registered trademarks of GE Healthcare.

Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.

LysoTracker is a registered trademark of Thermo Fisher Scientific.

References

1. Mol Ther (2016) 24(12), 2100-2108. doi:10.1038/mt.2016.179. (70058)
2. Carbohydrate Polym (2016) 153, 160-168. doi: 10.1016/j.carbpol.2016.07.080 (70058)
3. Leuk Res (2016) 42, 59-67. doi:10.1016/j.leukres.2016.01.007 (70058)
4. bioRxiv (2019) doi: https://doi.org/10.1101/747014 (70058)
5. Int J Pharm (2019) 565, 391-408. doi:10.1016/j.ijpharm.2019.05.009 (70058)
6. Oncogene (2019) 38, 1432–1447. https://doi.org/10.1038/s41388-018-0507-6 (70058)
7. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4 (70067, SIM)
8. Spectrochimica Acta A Mol Biomol Spectrosc (2020) 233, 118234.https://doi.org/10.1016/j.saa.2020.118234 (70058)

Product Attributes

Cellular localization Lysosomes
For live or fixed cells For live/intact cells
Assay type/options Long term staining (24-72h), No-wash staining, Real-time imaging
Cell permeability Membrane permeant
Colors Blue, Green, Orange, Far-red
Storage Conditions Store at -10 to -35 °C, Protect from light

Protocols

Additional information

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LysoView™ 405, LysoView™ 488, LysoView™ 540, LysoView™ 633, LysoView™ 650

Size

Trial size (10 uL), 50 uL, Trial Size (1 vial), Set of 10 vials

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