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Calcein (High Purity)

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Description

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12.

PRODUCT ATTRIBUTES
CAS number 154071-48-4
Cellular localization Fluid phase tracer
Cell permeability Membrane impermeant
Colors Green
Excitation/Emission 494/517 nm (pH 8)
PRODUCT DESCRIPTION

Calcein is a highly negatively charged, water-soluble fluorescein derivative. Its fluorescence is nearly independent of pH in the range between 6.5 and 12. The dye can be introduced into cells by microinjection and is useful for cell tracing and for the study of cell membrane integrity and fusion. Biotium also offers calcein AM, the membrane-permeant version of calcein that hydrolyzes into calcein once inside cells.

  • λExEm = 494/517 nm (pH 8)
  • ε = 75,000
  • Yellow-orange solid soluble in DMSO, DMF or pH>6 water
  • Store at 4°C and protect from light
  • C30H26N2O13
  • MW: 623
  • [154071-48-4]

Protocols

SDS

Additional information

Cat. No

80013

volume

100 mg

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volume

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