Description
Product description:
A chambered coverslip with 8 individual wells and high walls for cell culture, immunofluorescence, and high-end microscopy
- All-in-one 8 well chamber slide for cost-effective experiments with small cell numbers and low reagent volumes
- High-resolution imaging through No. 1.5 polymer coverslip bottom with the highest optical quality
- Suitable for various microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, and LSFM)
- Extra high individual walls to keep cross contamination between wells as low as possible
Applications:
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- Cultivation and high-resolution microscopy of cells
- Immunofluorescence staining and fluorescence microscopy of living and fixed cells
- Live cell imaging over extended time periods
- Transfection assays
- Differential interference contrast (DIC) microscopy when used with a DIC lid
Technical Features:
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- Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
- ibiTreat (tissue culture-treated) surface for optimal cell adhesion
- Imaging chamber slide with excellent optical quality for high-end microscopy
- Individual well walls for minimizing well-to-well crosstalk and contaminations
- Compatible with staining and fixation solutions
- Biocompatible plastic material—no glue, no leaking
- Also available as an adhesive version without a bottom: sticky-Slide 8 Well high
- Also available with a Glass Coverslip Bottom: µ-Slide 8 Well high Glass Bottom for special microscopic applications
- Additional version available with a 500 µm grid: µ-Slide 8 Well high Grid-500
- Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
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Specifications:
| Outer dimensions (w x l) | 25.5 x 75.5 mm² |
| Number of wells | 8 |
| Dimensions of wells (w x l x h) | 9.4 x 10.7 x 9.3 mm³ |
| Volume per well | 300 µl |
| Height with/without lid | 10.8/9.5 mm |
| Growth area per well | 1.0 cm² |
| Coating area per well2.2 | cm² |
| Bottom: ibidi Polymer Coverslip | |
Technical Drawing
Technical drawings and details are available in the Instructions (PDF).
The Principle of the µ-Slide 8 Well high
The Coverslip Bottom
The µ-Slide 8 Well high comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky-Slide 8 Well high without any bottom and the µ-Slide 8 Well high Glass Bottom for special microscopic applications.
Find more information and technical details about the coverslip bottom of the ibidi chambers here.
The ibiTreat Surface
ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.
Find more information about the different surfaces of the ibidi chambers here.
Application Examples:
Immunofluorescence
The ibidi µ-Slide 8 Well high allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy.
Live Cell Imaging
The µ-Slide 8 Well high enables high-resolution live cell imaging using different microscopy techniques.
Together with the ibidi
Stage Top Incubation System, you can keep your cells happy for a long time by precisely controlling temperature, humidity, and CO2 concentration on your microscope.
Want to know if you should use a glass or a polymer bottom for your application? Find out here.
Instructions
Application Guides
- AG 31: Microscopy With the ibidi Chambers (PDF)
- AG 41: Cell Culture and Preparation for Imaging (PDF)
- AG 39: Immunofluorescence Assays (PDF)
Application Notes
Product Flyer
FAQ
- FAQ: Cell Culture and Microscopy With the ibidi Labware
- Which solvents and chemicals are compatible with the ibidi labware?
- Which immersion oils are compatible with ibidi products?
Additional information
| Surface Modification | Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized, Collagen IV: #1.5 polymer coverslip, sterilized, Poly-L-Lysine: #1.5 polymer coverslip, sterilized, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized |
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| Pcs./Box | 15, 90 |