A simple, colorimetric assay for determining live cell numbers by absorbance on a microplate reader.
The XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers by absorbance on a microplate reader. XTT is a tetrazolium derivative that is turned into a water-soluble orange product after reduction by mitochondrial enzymes that are only present in metabolically active live cells. The amount of orange product generated is proportional to the number of living cells in the sample.
Determination of live cell numbers is often used to assess the rate of cell proliferation and cytotoxicity caused by drugs and cytotoxic agents. The non-radioactive MTT assay developed by Mossman is one of the most popular viability assays; however, the product produced is non-soluble, and requires a solubilization step. Unlike MTT, XTT is reduced to a highly water-soluble orange-colored product instead of the insoluble formazan from MTT, thus eliminating the solubilization step required for the MTT assay.
The amount of water-soluble product generated from XTT reduction is proportional to the number of living cells in the sample and can be photometrically quantified at 475 nm. Continuous color development permits measurements at multiple time points for extended detection range.
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|Apoptosis/viability marker||Metabolic activity|
|For live or fixed cells||For live/intact cells|
|Assay type/options||Endpoint assay, Real-time imaging, Homogeneous assay, High-throughput assay|
|Detection method/readout||Microplate reader (absorbance)|
|Storage Conditions||Store at 2 to 8 °C|