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Resazurin Cell Viability Assay Kit (alamarBlue™)

192.00 USD455.00 USD

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Description

     

Resazurin Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. This assay has excellent performance compared to other resazurin-based cell proliferation kits such as alamarBlue®, PrestoBlue®, or CellTiter-Blue®.

  • Single-step, homogeneous, high-throughput cell quantitation
  • For fluorescence- or absorbance-based measurement
  • Sensitive, requires as few as 80 cells for reproducible results
  • Widely used in cultured mammalian cells, bacteria, protozoa, yeast, & fungi
  • Cell lysis not required, allows long-term kinetic monitoring
  • Non-toxic, non-radioactive, non-carcinogenic, simple disposal
  • Ready-to-use solution

Resazurin detects cell metabolic by converting from a nonfluorescent dye to the highly red fluorescent dye resorufin in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the redox indicator to change from the oxidized form (nonfluorescent, blue) form to the reduced form (fluorescent, red).

Reduced resazurin can be detected using fluorescence (Ex/Em 530-560/590 nm) or absorbance at 570 nm. The fluorescent or colorimetric signal is proportional to the number of living cells in the sample. Results can obtained between 1-24 hours after adding substrate. Resazurin solution is supplied ready-to-use in 25 mL (2500 assay) or 100 mL (10,000 assay) sizes.

See our full selection of Cell Viability & Apoptosis Assays.

alamarBlue is a registered trademark of Trek Biosystems. CellTiter-Blue is a registered trademark of Promega Corporation. PrestoBlue is a registered trademark of Thermo Fisher Scientific

PRODUCT ATTRIBUTES

Apoptosis/viability marker Metabolic activity
For live or fixed cells For live/intact cells
Detection method/readout Microplate reader (absorbance), Microplate reader (fluorescence)
Assay type/options Endpoint assay, Real-time imaging, Homogeneous assay, High-throughput assay
Colors Red
Storage Conditions Store at 2 to 8 °C

protocol

PI-30025

SDS

SDS-30025

supporting documents

 

REFERENCES

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12. Antimicrob Agents Chemother (2016) 60(7), 4183–4196. doi:10.1128/AAC.03021-15
13. Curr Prot Toxicol (2016) 68, 2.24.1-2.24.15. doi: 10.1002/cptx.1
14. J Biol Chem (2016) 291, 21869-21879. doi: 10.1074/jbc.M115.712166
15. Curr Prot Stem Cell Biol (2018) 26(1), 5C.3.1-5C.3.19. DOI:10.1002/9780470151808.sc05c03s26
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Additional information

Cat. No

30025-1, 30025-2

volume

25mL, 100mL

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volume

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