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TUNEL Assay Apoptosis Detection Kit

509.00 USD711.00 USD

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Description

The CF® dye TUNEL Assay Apoptosis Detection Kit label apoptotic cells in fixed tissue sections or cellular samples for fluorescence microscopy or flow cytometry.

Product Description

These kits use enzymatic labeling of DNA double-strand breaks to fluorescently stain apoptotic cells in fixed samples or tissue sections.

Features

  • Direct labeling of DNA ds breaks with fluorescent dUTP
  • Bright & photostable next-generation CF® dyes
  • For fixed cells or tissue sections
  • Detect by fluorescence microscopy or flow cytometry

Kit Components

  • Equilibration Buffer
  • CF® Dye TUNEL Reaction Buffer
  • TdT Enzyme (calf thymus; recombinant, produced in E. coli)

Dye Options

  • Green fluorescent CF®488A (Ex/Em 490/515 nm)
  • Red fluorescent CF®594 (Ex/Em 593/614 nm)
  • Far-red fluorescent CF®640R (Ex/Em 642/662 nm)

Learn more about Biotium’s next-generation CF® Dyes.

In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3′- ends of the internucleosomal DNA strand breaks that are a hallmark of apoptosis. The CF® dye TUNEL Assay Apoptosis Detection Kits employs dUTP conjugated to the exceptionally bright and photostable fluorescent dyes, for single step fluorescent TUNEL labeling of fixed cells or tissues for analysis by fluorescence microscopy or flow cytometry.

See our full selection of Cell Viability & Apoptosis Kits.

 

References

1. PLoS ONE (2013) 8(1), e53657. doi:10.1371/journal.pone.0053657
2. Cell Death Diff (2014) 21, 397–406. doi:10.1038/cdd.2013.152
3. Nat Com (2014) 5, 4487. DOI: 10.1038/ncomms5487
4. Int J Ophthalmol (2016) 9(6), 825-30. doi: 10.18240/ijo.2016.06.06
5. Future Med Chem (2017) 9(17), 2011-2028. doi: 10.4155/fmc-2017-0118
6. Genetics (2017) 205, 707–723. doi: 10.1534/genetics.116.190850
7. Nat Com (2017) 8, 14328. DOI: 10.1038/ncomms14328
8. PLoS ONE (2017) 12(7), e0180695. https://doi.org/10.1371/journal.pone.0180695
9. Sci Rep (2017) 7, 14061. DOI:10.1038/s41598-017-14444-3 1
10. Stem Cell Rep (2017) 8, 264–277. http://dx.doi.org/10.1016/j.stemcr.2016.12.021
11. FASEB J (2018) 32(4), 1957-1968. doi: 10.1096/fj.201700871R
12. JCI Insight (2018) 3(15), e121017. https://doi.org/10.1172/jci.insight.121017
13. J Endocrinol (2018) 236, 151–165. https://doi.org/10.1530/JOE-17-0613
14. J Enz Inhib Med Chem (2018) 33(1), 434-444. DOI: 10.1080/14756366.2017.1419216
15. Sci Rep (2018) 8, 10876. DOI:10.1038/s41598-018-29118-x
16. eLife (2019) 8, e47097. DOI: https://elifesciences.org/articles/47097

Product Attributes

Apoptosis/viability marker DNA fragmentation
For live or fixed cells For fixed cells/tissue sections
Detection method/readout Fluorescence microscopy, Flow cytometry
Assay type/options Endpoint assay
Fixation options Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)
Colors Green, Red, Far-red
Product origin TdT enzyme (calf thymus); recombinant, produced in E. coli
Storage Conditions Store at -10 to -35 °C, Protect from light

Additional information

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CF®488A, CF®594, CF®640R

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