1×106 cells / 1.0 ml
|Description||Hepatocytes are the chief functional cells of the liver and perform an astonishing number of metabolic, endocrine, and secretory functions. They are cuboidal epithelial cells, and 70–85% of the liver volume is occupied by hepatocytes.
Human hepatocytes are routinely used by the pharmaceutical industry for study of hepatotoxicity, drug clearance, and drug-drug interactions. However, their use is largely impeded by inadequate supply, high cost, high variability, and limited in vitro proliferation capacity. Alternative cell sources include hepatic cell lines and stem-cell derived hepatocytes but most of them do not exhibit sufficient in vivo-like functionality to be of pharmaco-toxicological relevance. In this scenario, immortalized human hepatocytes serve as the optimal in vitro model of the human liver as they are more easily maintained, propagated in culture, and have less intrinsic variation. They provide a valuable tool for investigating metabolic pathways and hepatocyte proliferation-related events.
|Species||Human (H. sapiens)|
|Seeding Density||5 – 15,000 cells/cm2; Recommended split ratio of 1:2 to 1:5*
* Do not exceed a split ratio of 1:5
|Population Doubling Time||30 – 35 hours|
|Immortalization Method||Serial passaging and transduction with recombinant lentiviruses carrying HPV E6/E7 gene|
|Applications||For Research Use Only|
|Unit quantity||1×106 cells / 1.0 ml|
|Cell Type||Immortalized Cells|
|Propagation Requirements||Grow cells in culture vessels coated with Applied Cell Extracellular Matrix (G422). Coat plates at 37.0°C overnight and wash
with sterile PBS prior to use.
The base media for this cell line is Prigrow IX medium available at?abm?Cat. No.?TM019. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.