• One-Step Protein Gel Stains

Low-cost, rapid, non-toxic protein gel stains

  • One-Step Blue®

Alternative to tedious Coomassie blue staining

  • One-Step Lumitein™ & One-Step Lumitein™ UV

Replacements for costly and time consuming SYPRO Ruby and Oriole stains

Our One-Step protein gel stains are low-cost, quick, convenient and easy-to-use for protein gel (SDS-PAGE) staining. Staining is quick: 5 to 60 min in a single step without fixation or washing. These stains have non-toxic formulations for safer handling and easy disposal. One-Step stains are certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

The One-Step stains are compatible with Edman-based sequencing and with mass spectrometry.

  • 100% H2O-based formulation
  • NO stinky acetic acid
  • NO toxic methanol
  • NO microwaving
  • NO washing
  • NO fixing
One-Step Blue® is an alternative to tedious staining protocols using Coomassie blue dye, and can be imaged visually or using a near-infrared fluorescence imager.
Red fluorescent One-Step Lumitein™ is an alternative to time-consuming and expensive SYPRO® Ruby protein gel stain, and it can be imaged on a UV box or laser gel scanner
One-Step Lumitein™ UV Gel Stain is an alternative to Oriole™ and Flamingo™ Gel Stains. Best stain for UV transilluminator. It does not contain hazardous solvents or shrink gels.

Product Information

Product Replacement for Catalog number Unit size Product protocol Safety report
One-Step Blue® Coomassie stain 21003-1L 1L PI-21003 One-Step Gel Stains Safety Report
One-Step Lumitein™ SYPRO® Ruby Protein Gel Stain 21004-1L 1L PI-21004
21004-4L 4L
One-Step Lumitein™ UV Oriole™ and Flamingo™ Protein Gel Stains 21005-1L 1L PI-21005
21005-4L 4L

One-Step Blue is a registered trademark of Biotium.

Lumitein and its related technologies are covered by US and international patents.
SYPRO is a registered trademark of Molecular Probes, Inc.
Oriole and Flamingo are trademarks of Bio-Rad Laboratories.