Mini Cart

µ-Slide 8 Well

142.80 $714.00 $

Surface Modification

Pcs./Box

Clear

Description

Product with ibidi Polymer Coverslip #1.5 bottom Optimized for inverted microscopy Suitable for adherent cells Suitable for immunofluorescence assays

 

A chambered coverslip with 8 wells for cell culture, immunofluorescence, and high-end microscopy

  • All-in-one 8 well chamber slide for cost-effective experiments—small number of cells and low volume of reagents needed
  • In this microscopy slide, the cells are imaged on a No. 1.5 polymer coverslip bottom with the highest optical quality
  • A cell culture chamber suitable for most microscopy techniques (e.g., DIC, widefield fluorescence, confocal microscopy, two-photon microscopy, FRAP, FRET, FLIM, or LSFM)

80826

Applications

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Outer dimensions (w x l) 25.5 x 75.5 mm²
Number of wells 8
Dimensions of wells (w x l x h) 9.4 x 10.7 x 6.8 mm³
Volume per well 300 µl
Total height with lid 8 mm
Growth area per well 1.0 cm²
Coating area per well 2.20 cm²
Bottom: ibidi Polymer Coverslip

Define and print your experimental setup

Technical Features

  • Chambered coverslip with 8 independent wells and a non-removable polymer coverslip-bottom
  • ibiTreat (tissue culture-treated) surface for optimal cell adhesion
  • Imaging chamber slide with excellent optical quality for high-end microscopy
  • Closely fitting lid
  • Compatible with staining and fixation solutions
  • Biocompatible plastic material—no glue, no leaking
  • Also available as an adhesive version without a bottom: sticky-Slide 8 Well
  • Also available with a Glass Coverslip Bottom: µ-Slide 8 Well Glass Bottom for special microscopic applications
  • Additional version available with a 500 µm grid: µ-Slide 8 Well Grid-500

The Principle of the µ-Slide 8 Well

The Coverslip Bottom

The µ-Slide 8 Well comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as a sticky version without any bottom, or and as an option with a Glass Coverslip Bottom for special microscopic applications.

Find more information and technical details about the coverslip bottom of the ibidi chambers here.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Find more information about the different surfaces of the ibidi chambers here.

Application Examples

Immunofluorescence

The ibidi µ-Slide 8 Well allows for standard immunofluorescence protocols to be employed without the use of coverslips in an all-in-one chamber. All steps (e.g., cell cultivation, fixation, staining, and imaging) are carried out in the open well geometry. After staining, the sample can be observed through the coverslip bottom using high-resolution microscopy. The closely fitting lid prevents evaporation and allows for long-term assays.

Fluorescence microscopy of MDCK cells. Mitochondria (MitoTracker, red), Actin cytoskeleton (Phalloidin, green), nuclei (DAPI, blue).

open_well1.jpg

Live Cell Imaging

The µ-Slide 8 Well enables high-resolution live cell imaging using different brightfield and fluorescence techniques. Here, live cell microscopy was performed using the µ-Slide 8 Well in the ibidi Heating System, Universal Fit, for 1 Chamber on a Nikon Eclipse TIE inverted microscope.

Live cell imaging in a µ-Slide 8 Well using transmitted light.

Live cell imaging in a µ-Slide 8 Well using fluorescence.

F-actin Visualization in Living Cells Using LifeAct-TagGFP2 Protein
Live cell imaging of F-actin in Rat1 fibroblasts after LifeAct-TagGFP2 Protein transfer (30 µg/ml, 3 minutes) using the µ-Slide 8 Well.

Live Cell Imaging of Algae
Brightfield microscopy of the green freshwater algae Haematococcus pluvialis in a µ-Slide 8 Well. The cells are in an immature cyst state with developing centers, filled with the strong antioxidant astaxanthin (red), and surrounded by Chlorophyll from the chloroplasts (green). 40x objective lens.

Live Cell Imaging of Fibroblasts After Transfection
Live cell imaging on an inverted widefield fluorescence microscope. Rat fibroblast cells 24 hours after transfection with pCMV-eGFP. Red: Rhodamine-labelled fluorescent lipoplexes, Green: eGFP fluorescence. 60x objective lens.

TA_DIC_Rat1.jpg

DIC Microscopy of Mammalian Cells
Differential interference contrast (DIC) microscopy of mammalian cell culture (fibroblasts) using the µ-Slide 8 Well and the DIC lid for µ-Slides. 63x objective lens.

Additional information

Surface Modification

Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized, ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized, Collagen IV: #1.5 polymer coverslip, sterilized, Poly-L-Lysine: #1.5 polymer coverslip, sterilized

Pcs./Box

15, 90

Supporting Material

Instructions

Application Notes

Movies

Product Flyer

FAQ

User Comments

Wenting You, University of Maastricht, The Netherlands

“I tested various slides and found that the µ-Slide 8 Well worked very well. I use the slides for confocal microscopy imaging. My cells are very easy to detach from glass bottom cell culture slides. Therefore, I tried the slides with a Polymer Coverslip bottom. The cells adhere to the cell culture slide very well, and I can get high-resolution images. I have already ordered more polymer cell culture slides from ibidi.”

Wenting You
University of Maastricht
The Netherlands
https://cris.maastrichtuniversity.nl/portal/en/

Johanna Hol Fosse, Norwegian Veterinary Institute, Oslo, Norway

I have tested the µ-Slides 8 Well on adherent fish cell lines, originally isolated from salmon head kidney (ASK and SHK cells) and have been very happy with them. The ASK cells preferred the ibiTreat slides, while the SHK cells grew well on both ibiTreat and Collagen IV. I also tested the ASK cells in a µ-Slide 18 Well – Flat and that worked well, provided that the medium (L-15, 10% FCS) was changed at least every other day. Cells were seeded 5,000/well in the µ-Slide 18 Well – Flat and 50,000/well in the µ-Slide 8 Well. I will place an order within the next few weeks.”

Johanna Hol Fosse
Norwegian Veterinary Institute
Oslo
Norway
https://www.vetinst.no/en

Yuki Manabe, Kyoto University, Graduate School of Agriculture, Kyoto, Japan

“Thank you for your great product µ-Slide 8 Well. I think it is the best for obtaining fluorescent images of adherent cells such as RAW264 and RBL-2H3 using oil immersion microscopy. Thanks to this slide, we can get various good results.”

Yuki Manabe
Kyoto University
Graduate School of Agriculture
Kyoto
Japan

https://www.kais.kyoto-u.ac.jp/english/

Michelle Somerville, Cardiff University, Cardiff, United Kingdom

“We have tested the µ-Slide Angiogenesis and the µ-Slide 8 Well and have found them to be great products. The slides are being used for imaging fluorescently labelled organoids using confocal microscopy and we will continue to use them. I have already recommended them to a lab colleague and will continue to recommend them to others.”

Michelle Somerville
Cardiff University
Cardiff
United Kingdom

https://www.cardiff.ac.uk/

Lee Shu Ying, National University of Singapore, Singapore

“As a microscopy core facility, we have to recommend the most suitable imaging plates to our users for their experiments. The µ-Slide 8 Well is our best seller amongst the other wonderful platforms that ibidi carries.

We had multiple experiments where the cells/bacteria were not adhering well to glass coverslips, but grew perfectly on the ibiTreat surface. Confocal images turned out great as well. Our users also love how the cover fits snugly for ease of handling.”

Lee Shu Ying
National University of Singapore
Yong Loo Lin School of Medicine
Singapore

http://nusmedicine.nus.edu.sg/medical-sciences-cluster/confocal-microscopy-unit/

Madeleine Zerbato, Brigham Regenerative Medicine Center, Cambridge, USA

“The ibidi µ-Slide 4 Well and µ-Slide 8 Well that I am working with are actually the best products I have tested so far for cultivating D3 mouse embryonic cell lines. They attach well to the treated slide (ibiTreat µ-Slides), and the resulting morphology is very similar to the one they have on regular plastic. That is something very unique to those microscopic slides, since D3 mESCs do not attach to glass and form embryoid-like structures. I tested other non-glass slides from other manufacturers but the ibiTreat µ-Slides are the only ones that give me a physiological-like morphology.”

Madeleine Zerbato
Brigham Regenerative Medicine Center
Cambridge, USA

http://brmc.bwh.harvard.edu/

Jessica Davis, University College Dublin, Ireland

“I used the µ-Slide 8 Well in the glass version and in the plastic version with ibiTreat. They were perfect for my experiment—I was performing live cell imaging on 3D cell culture. I found that the Matrigel adhered very well to both slides. The coverslip thickness of the base resulted in superb imaging. Additionally, I noticed that the lid was very secure compared with other chamber slides I have used in the past. I am very happy with the product and have recommended it to other colleagues.”

Jessica Davis
University College Dublin
Ireland

http://www.ucd.ie/

Stefanie Kirchberger, Children’s Cancer Research Institute, Vienna, Austria

“We tested the µ-Slide 2 Well and µ-Slide 8 Well in the uncoated version for the confocal microscopy of fish larvae. Both types of slides worked fine on our confocal microscope. We have already ordered another bunch of those slides for the lab!”

Stefanie Kirchberger
Children’s Cancer Research Institute
Vienna, Austria

http://science.ccri.at/

Ivan Bedzhov, University of Cambridge, Gurdon Institute, Cambridge, United Kingdom

“We looked for an imaging-compatible material that supports embryo attachment. This led us to establish that we can successfully grow and image mouse embryos on ibiTreat μ-Plates 24 Well and µ-Slides 8 Well from ibidi, which have optical properties similar to glass, and allow efficient blastocyst attachment. Thus, ibidi slides and plates are now an indispensable part of our in vitro culture methodology.“

Ivan Bedzhov
University of Cambridge
Gurdon Institute
Cambridge
United Kingdom

http://www.gurdon.cam.ac.uk/

Owen McCarty, PhD, Oregon Health & Science University, Portland, USA

“The ibidi platforms have opened new possibilities for our group to utilize small volumes of blood to characterize the cell biology of platelets and white blood cells. Moreover, we have greatly benefited from collaborating with the technical representatives at ibidi to discuss ways to multiplex the ibidi platforms to address our experimental questions.”

Owen McCarty, PhD
Associate Professor
Department of Biomedical Engineering
Oregon Health & Science University
Portland, Oregon
USA

http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/biomedical-engineering/

Joanne Marrison, University of York, UK

“I plated and transfected cells on the ibidi µ-Slide 8 Well alongside the regular chambers we normally use and they were very good. In fact, I particularly liked the lids which seem more secure than our regular 8-well chambers and the fact that they are ‘slide’ sized with space for writing a label. Another bonus was the individual packaging which gives me greater confidence on sterility particularly when not all the chambers in a pack of 8 are used at once. So all in all great.”

Joanne Marrison
Imaging and Cytometry Laboratory (B/K051)
Bioscience Technology Facility, Department of Biology
University of York, York
United Kingdom

www.york.ac.uk/biology/tf

Prof. Dr. Markus Sauer, University of Wuerzburg, Germany

„ibidi slides are compelling!
We tested the open μ-Slides with 8 wells for live-cell super-resolution imaging by dSTORM with SNAP-tags. The quality of the super-resolved images was impressive.“

Prof. Dr. Markus Sauer
Biotechnology & Biophysics
Julius-Maximilians-University Wuerzburg
Germany

http://www.super-resolution.biozentrum.uni-wuerzburg.de/startseite/

Movies

MV 26: Cell Culture and Microscopy Using the µ-Slide 8 Well

Reviews (0)

Write a review

Surface Modification

Pcs./Box

Clear