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This ultra high sensitivity DNA quantitation assay can detect ultra low amounts of DNA better than any competitor kit on the market
A versatile, highly sensitive assay with an extremely broad dynamic range
AccuGreen™ High Sensitivity and Broad Range kits are affordable alternatives to Qubit® dsDNA kits
Wide linear range of 2-2000 ng
Non-mutagenic dye for safer handling & easy disposal
RNA-selective, sensitive assay for plate reader or Qubit®. The widest dynamic range, equal to Quant-iT™ HS and BR combined
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. Some of our kits offer unrivaled sensitivity or linear ranges. To choose the DNA quantitation assay that is right for you, see the linear detection ranges and features table below.
Microplate reader assay features
- Allow quantitation of many samples and replicates at once, for better accuracy
- Microplate readers have the highest sensitivity and adjustable excitation and emission settings
- The AccuBlue® NextGen assay is the most sensitive DNA quantitation assay on the market, allowing accurate detection of 1 pg* of DNA
- The AccuClear® assay is extremely versatile, with a very broad dynamic range, allowing quantitation of nearly any sample
Qubit® reader assay features
- AccuGreen™ assay kits designed for use in the pre-programmed dsDNA quantitation assays on the Qubit® reader
- AccuGreen™ assay kits are direct replacements for the Qubit® assay kits
- Significant cost savings when compared to the Qubit® kits
All DNA Quantitation Kits
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Suggested Instrument | Features |
|---|---|---|---|---|
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen | 1 pg-3 ng* | 468/507 | Fluorescent Plate Reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity |
| AccuClear® Ultra High Sensitivity | 30 pg-250 ng | 468/507 | Fluorescent Plate Reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity | 0.2-100 ng | 485/530 | Fluorescent Plate Reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range | 2-2000 ng | 350/460 | Fluorescent Plate Reader | Broad linear range with blue fluorescence |
ACCUBLUE® NEXTGEN DNA QUANTITATION
- Linear range: 1-3000 pg dsDNA (depending on plate reader capability)
- Unrivaled sensitivity for ultra low amounts of DNA
- Working solution stable for up to 6 months in the fridge for added convenience
- Green fluorescence (Ex/Em: 460/507 nm)
- Dye is a perfect match for blue LED excitation sources
- Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
- Available with or without calf thymus dsDNA standard
Click here to download an App Note on using the AccuBlue® NextGen assay on the BMG CLARIOStar® to quantify extremely low amounts of DNA.
AccuBlue® NextGen Products
| Product | DNA Standards | Catalog No. | Size |
|---|---|---|---|
| AccuBlue® NextGen dsDNA Quantitation Kit | 10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution | 31060-T | Trial size (200 assays) |
| 31060 | 1000 assays | ||
| AccuBlue® NextGen dsDNA Quantitation Solution | None, for use with DNA standard of your choice | 31061-T | Trial size (200 assays) |
| 31061 | 1000 assays |
ACCUCLEAR® ULTRA HIGH SENSITIVITY DNA QUANTITATION
- Linear range: 0.03-250 ng dsDNA
- Very high sensitivity and dynamic range
- Green fluorescence (Ex/Em: 468/507 nm)
- Dye is a perfect match for blue LED excitation sources
- Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor™-P, or Nanodrop® Spectrofluorometer
- Available with or without calf thymus DNA standards
AccuClear® Products
| Product | DNA Standards | Catalog No. | Size |
|---|---|---|---|
| AccuClear® Ultra High Sensitivity dsDNA Quantitation Kit with DNA Standards | 25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution | 31028-T | Trial size (200 assays) |
| Set of 7 prediluted calf thymus dsDNA standards | 31028 | 1000 assays | |
| 25 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution | 31029 | 2000 assays | |
| AccuClear® Ultra High Sensitivity dsDNA Solution | None, for use with DNA standard of your choice | 31027-T | Trial size (200 assays) |
| 31027 | 1000 assays | ||
| AccuClear® dsDNA Standard, 25 ng/uL | Standalone DNA standard, 25 ng/uL | 31029C | 1 mL |
ACCUBLUE® HIGH SENSITIVITY DNA QUANTITATION
- Linear range: 0.2-100 ng dsDNA
- Non-toxic, non-mutagenic dye for safer handling and easy disposal
- Green fluorescence (Ex/Em: 500/530 nm)
- Designed for fluorescence microplate reader, spectrally compatible with Qubit®, QuantiFluor-P®, or Nanodrop® Spectrofluorometer
- Available with or without calf thymus dsDNA standards
- Set of standards also available separately
AccuBlue® High Sensitivity Products
| Product | DNA Standards | Catalog No. | Size |
|---|---|---|---|
| AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards | 10 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution | 31006-T | Trial size (200 assays) |
| Set of 8 prediluted calf thymus dsDNA standards | 31006 | 1000 assays | |
| AccuBlue® High Sensitivity dsDNA Solution | None, for use with DNA standard of your choice | 31008-T | Trial size (200 assays) |
| 31008 | 1000 assays | ||
| AccuBlue® High Sensitivity dsDNA Standards, Set of Eight | Stand-alone set of standards (0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL) | 31006C | 0.5 mL each |
ACCUGREEN™ DNA QUANTITATION KITS FOR QUBIT®
AccuGreen™ High Sensitivity
- Designed specifically for Thermo’s Qubit® fluorometer
- Linear range: 0.1-100 ng dsDNA
- Green fluorescence (Ex/Em: 502/523 nm)
- Replaces Qubit® dsDNA HS Assay Kit for cost savings
AccuGreen™ Broad Range
- Designed specifically for Thermo’s Qubit® fluorometer
- Linear range: 2-1000 ng dsDNA
- Green fluorescence (Ex/Em: 500/530 nm)
- Replaces Qubit® dsDNA BR Assay Kit for cost savings
AccuGreen™ Products
| Product | DNA Standards | Catalog No. | Size |
|---|---|---|---|
| AccuGreen™ High Sensitivity dsDNA Quantitation Kit (for Qubit®) | 0 & 10 ng/uL calf thymus dsDNA | 31066-T | Trial size (100 assays) |
| 31066 | 500 assays | ||
| AccuGreen™ High Sensitvity dsDNA Quantitation Solution (for Qubit®) | None, for use with DNA standard of your choice | 31068-T | Trial size (100 assays) |
| 31068 | 500 assays | ||
| AccuGreen™ Standard 2, 10 ng/uL | Standalone DNA standard, 10 ng/uL | 99820-T | 1 mL |
| AccuGreen™ Broad Range dsDNA Quantitation Kit (for Qubit®) | 0 & 100 ng/uL calf thymus dsDNA | 31069-T | Trial size (100 assays) |
| 31069 | 500 assays | ||
| AccuGreen™ Broad Range dsDNA Quantitation Solution (for Qubit®) | None, for use with DNA standard of your choice | 31070-T | Trial size (100 assays) |
| 31070 | 500 assays | ||
| AccuGreen™ Standard 2, 100 ng/uL | Standalone DNA standard, 100 ng/uL | 99838-T | 1 mL |
ACCUBLUE® BROAD RANGE DNA QUANTITATION
- Linear range: 2-2000 ng dsDNA
- Blue fluorescence emission (Ex/Em: 350/460 nm)
- For fluorescence microplate reader
- Available with or without calf thymus dsDNA standards
- Set of standards also available separately
AccuBlue® Broad Range DNA Quantitation Products
| Product | DNA Standards | Catalog No. | Size |
|---|---|---|---|
| AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards | 200 ng/uL calf thymus dsDNA with instructions for preparing standard curve by dilution | 31007-T | Trial size (200 assays) |
| Set of 9 prediluted calf thymus dsDNA standards | 31007 | 1000 assays | |
| AccuBlue® Broad Range dsDNA Solution | None, for use with DNA standard of your choice | 31009-T | Trial size (200 assays) |
| 31009 | 1000 assays | ||
| AccuBlue® Broad Range dsDNA Standards, Set of Nine | Stand-alone set of standards, 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL | 31007C | 0.5 mL each |
ACCUBLUE® RNA QUANTITATION KIT
AccuBlue® RNA Quantitation Kit Details
- Linear range of 5-1000 ng RNA
- Far-red fluorescence emission (Ex/Em: 630/660 nm)
- Optimized for fluorescence microplate readers with far-red fluorescence
- Optimized for the Qubit® 3 pre-programmed RNA BR program
- Comes with a mammalian-derived total RNA standard
AccuBlue® RNA Quantitation Kit Advantages
- Selective: Can tolerate equivalent amounts of dsDNA (see Figure below)
- Convenient: Wide linear range spans Qubit®/Quant-iT™ HS and BR ranges
- Flexible: For fluorescence microplate reader OR small fluorometer like Qubit®
- Accurate: The mammalian-derived total RNA standard provides the highest accuracy when quantifying samples from similar sources
RNA Quantitation Kits
| Product | Catalog No. | Size |
|---|---|---|
| AccuBlue® Broad Range RNA Quantitation Kit | 31073-T | Trial size (200 assays) |
| AccuBlue® Broad Range RNA Quantitation Kit | 31073 | 1000 assays |
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit, Quant-iT, PicoGreen and NanoDrop are trademarks of Thermo Fisher Scientific.