HIGH-PERFORMANCE CF® DYES

 

Superior brightness, photostability, and solubility

MULT-COLOR FLEXIBILITY

 

30 CF® dyes across the visible, far-red & near-IR spectra
UNRIVALED NEAR-IR DYES

 

For NIR western or small animal imaging
SUPER-RESOLUTION MICROSCOPY

 

The best performance for STORM, SIM, and other methods
 
EXCELLENT LABELING EFFICIENCY

More stable succinimidyl esters

WIDE SELECTION OF PRODUCTS

Antibodies, conjugates, reactive dyes and labeling kits

 
 
CF® Dye Spectrum:
 
    CF®350 Dye

    CF®350 is a blue fluorescent dye

Technical SummaryAbs/Em Maxima: 347/448 nm
Extinction coefficient: 18,000
Molecular weight: ~ 496
Excitation source: UV
Replaces: Alexa Fluor® 350, AMCA,
DyLight® 350
 
Features
Brighter and more photostable than AMCA
Direct replacement for Alexa Fluor® 350
Highly water soluble and pH-insensitive

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    CF®405S Dye

    Improved brightness & photostability for the 405 nm laser line

Technical SummaryAbs/Em Maxima: 404/431 nm
Extinction coefficient: 33,000
Molecular weight: ~ 1,169
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 405, Cascade Blue®,
DyLight® 405
 
Features
Brighter than Alexa Flour®  408

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    CF®405M Dye

    Improved brightness & photostability for the 405 nm laser line

Technical SummaryAbs/Em Maxima: 408/452 nm
Extinction coefficient: 41,000
Molecular weight: ~ 503
Excitation laser line: 405 nm
Replaces: Pacific Blue®, BD Horizon™ V450
 
Features
More photostable than Pacific Blue®, with less spill-over in the green channel
An excellent choice for super-resolution imaging by SIM

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    CF®405L Dye

    A 405 nm-excitable dye with orange fluorescence emission

      Technical Summary

     Abs/Em Maxima: 395/545 nm
     Extinction coefficient: 24,000
     Molecular weight: ~1573
     Excitation laser line: 405 nm
     Replaces: Pacific Orange®

Features

View Technology

 
    CF®430 & CF®440 Dyes

    Photostable 405 nm-excitable dyes with green fluorescence

Technical SummaryCF®430
Abs/Em Maxima: 426/498 nm
Extinction coefficient: 40,000
Molecular weight: ~429
Excitation laser line: 405 nm
Replaces: Pacific Green®, BD Horizon™ V500,
Krome Orange™
CF®440
Abs/Em Maxima: 440/515 nm
Extinction coefficient: 40,000
Molecular weight: ~716
Excitation laser line: 405 nm
Replaces: Alexa Fluor® 430
 
Features
Photostable dyes suitable for microscopy
Perfect match for the CFP filter set
Suitable for flow cytometry in the AmCyan channel
Highly water soluble and pH-insensitive

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    CF®450 Dye

    A green fluorescent dye with unique spectral properties

Technical SummaryAbs/Em Maxima: 450/538 nm
Extinction coefficient: 40,000
Molecular weight: ~689
Excitation laser line: 405 nm
 
Features

View Technology

 
    CF®488A Dye

    A superior green fluorescent dye

Technical SummaryAbs/Em Maxima: 490/515 nm
Extinction coefficient: 70,000
Molecular weight: ~914
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 488, DyLight® 488,
like FITC, FAM, Cy®2
 
Features
Minimally charged, for less non-specific binding than Alexa Fluor® 488
Narrower emission spectrum for less bleed to red
Very photostable
Compatible with super-resolution imaging by TIRF
Highly water soluble and pH-insensitive

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    CF®514 Dye

    Alternative green fluorescent dye

Technical SummaryAbs/Em Maxima: 516/548 nm
Extinction coefficient: 105,000
Molecular weight: ~1216
Excitation laser line: 488 nm
Replaces: Alexa Fluor® 514
 
Features
Image using the same settings as FITC or CF®488A
Can be distinguished from CF®488A in the same specimen by spectral imaging and linear unmixing

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    CF®532 Dye

    A bright orange fluorescent dye for the 532 nm laser

Technical SummaryAbs/Em Maxima: 527/558 nm
Extinction coefficient: 96,000
Molecular weight: ~ 685
Excitation laser line: 532 nm
Direct replacement for: Alexa Fluor® 532, Atto 532
 
Features
Designed for the 532 nm laser
Brighter than Alexa Fluor® 532
Highly water-soluble and pH-insensitive

View Technology

 
    CF®535ST Dye

    An orange fluorescent dye designed for STORM super-resolution imaging

Technical SummaryAbs/Em Maxima: 535/568 nm
Extinction coefficient: 95,000
Molecular weight: ~728
Excitation laser line: 532 nm
Features

View Technology

 
    CF®543 Dye

    An orange fluorescent dye ideal for the 543 nm laser

Technical SummaryAbs/Em Maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~ 870
Excitation laser line: 532 nm, 543 nm, or
546 nm
Direct replacement for: Alexa Fluor® 546,
TAMRA
 
Features
Optimized for the 543 nm laser
Yields the brightest conjugates among spectrally similar dyes
Highly water-soluble and pH-insensitive

View Technology

 
   CF®550R Dye
Technical Summary
Features

View Technology

 
    CF®555 Dye

    A bright and photostable orange-red dye

Technical SummaryAbs/Em Maxima: 555/565 nm
Extinction coefficient: 150,000
Molecular weight: ~ 901
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor®
555, ATTO 550, Cy®3, DyLight® 549,
Rhodamine
 
Features
Brighter than Cy®3
Highly water-soluble
Validated in multicolor STORM super-resolution imaging

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   CF®568 Dye

   Outshines Alexa Fluor®568

Technical SummaryAbs/Em Maxima: 562/583 nm
Extinction coefficient: 100,000
Molecular weight: ~ 714
Excitation laser line: 532 nm or 568 nm
Direct replacement for: Alexa Fluor® 568,
ATTO 565, Rhodamine Red
 
Features
Yields much brighter antibody conjugates than Alexa
Fluor® 568
Extremely photostable
Excellent choice for multi-color imaging with CF®488A and CF®640R
Compatible with TIRF and multicolor STORM super resolution imaging

View Technology

 
    CF®570 & CF®583 Dyes

    Bright red fluorescent dyes 

Technical Summary

CF®570 Technical Summary

Abs/Em maxima: 568/591 nm
Extinction coefficient: 150,000
Molecular weight: ~2998
Excitation laser line: 561 to 568 nm
Replaces: Alexa Fluor® 568, ATTO 565, DY-560, Rhodamine Red

CF®583 Technical Summary

Abs/Em maxima: 583/606 nm
Extinction coefficient: 150,000
Molecular weight: ~3127
Excitation laser line: 561 to 568 nm
Replaces: Cy®3.5

Features

View Technology

 
    CF®594 Dye

    Truly the brightest deep red dye

Technical SummaryAbs/Em Maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~730
Excitation laser line: 532 nm, 568 nm or
594 nm
Replaces: Alexa Fluor® 594, DyLight® 594,
Texas Red®
 
Features
Yields the brightest antibody conjugates among spectrally
similar dyes.
Excellent choice for multicolor imaging with green dyes like CF®488A
Extremely photostable
Also see CF®594ST, a version of CF®594 engineered specifically for STORM microcopy

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   CF®594ST Dye

   Deep red dye for STORM

Technical SummaryAbs/Em maxima: 593/614 nm
Extinction coefficient: 115,000
Molecular weight: ~868
Excitation laser line: 532 to 568 nm
Features

View Technology

 
    CF®620R Dye

    A bright and photostable far-red dye

Technical SummaryAbs/Em Maxima: 617/639 nm
Extinction coefficient: 115,000
Molecular weight: ~ 738
Excitation laser line: 633 nm or 635 nm
Replaces: LightCycler® Red 640
 
Features
Highly water-soluble
Highly fluorescent and extremely photostable
Absorption/emission at 617/639 nm for use in FRET or multi-color detection

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    CF®633 Dye

    The best dye for 633/635 laser lines

Technical SummaryAbs/Em Maxima: 630/650 nm
Extinction coefficient: 100,000
Molecular weight: ~820
Excitation laser line: 633 nm or 635 nm
Replaces: Alexa Fluor® 633, Alexa Fluor®
647, Cy®5, DyLight® 633, DyLight® 649
 
Features
Yields the brightest antibody conjugates among spectrally similar dyes
Far more photostable than Alexa Fluor® 647
Compatible with TIRF, FIONA, and gSHRImP super-resolution imaging methods

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    CF®640R Dye

    A highly photostable far-red dye

Technical SummaryAbs/Em Maxima: 642/662 nm
Extinction coefficient: 105,000
Molecular weight: ~ 832
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces Alexa Fluor® 647, ATTO 647N,
Cy®5, DyLight® 649
 
Features
Best photostability among Cy®5-like dyes
Yields highly fluorescent protein conjugates
Compatible with TIRF and FLIMP super-resolution microscopy

View Technology

 
    CF®647 Dye

    A highly fluorescent far-red dye

Technical SummaryAbs/Em Maxima: 650/665 nm
Extinction coefficient: 240,000
Molecular weight: ~ 1058
Excitation laser line: 633 nm, 635 nm or
640 nm
Replaces: Cy®5, Alexa Fluor® 647,
DyLight® 649
 
Features
Brighter than Cy®5
Highly water soluble and pH insensitive
Validated in multi-color super-resolution imaging by STORM

View Technology

 
    CF®660C and CF®660R Dyes

    Superior alternatives to Alexa Fluor® 660

Technical SummaryCF®660C
Abs/Em Maxima: 667/685 nm
Extinction coefficient: 200,000
Molecular weight: ~ 3112
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APCCF®660R
Abs/Em Maxima: 663/682 nm
Extinction coefficient: 100,000
Molecular weight: ~ 888
Excitation laser line: 633 nm, 635 nm or 640 nm
Replaces: Alexa Fluor® 660, APC
 
Features
CF®660C Features
Much brighter and more photostable than Alexa Fluor® 660
Compatible with multicolor super-resolution imaging by STORMCF®660R Features
Brighter than Alexa Fluor® 660
Unrivaled photostability among spectrally similar dyes

View Technology

 
    CF®680 and CF®680R Dyes

    Two outstanding 680 nm-excitable dyes

Technical SummaryCF®680
Abs/Em Maxima: 681/698 nm
Extinction coefficient: 210,000
Molecular weight: ~ 3241
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680CF®680R
Abs/Em Maxima: 680/701 nm
Extinction coefficient: 140,000
Molecular weight: ~ 912
Excitation laser line: 680 nm or 685 nm
Replaces: Alexa Fluor® 680, Cy®5.5, IR®Dye 680
 
Features
CF®680 Features
The brightest among spectrally similar dyes
Validated in multicolor STORM and 3D super-resolution
microscopy
Compatible with LI-COR® Odyssey®CF®680R Features
Unrivaled photostability among spectrally similar dyes
Compatible with STED and single molecule spectroscopy super-resolution imaging (see pp. 20-21)
Molecular weight compatible with nucleic acid labeling
Compatible with LI-COR® Odyssey®

View Technology

 
    Near-Infrared CF® Dyes

    Choose from six unrivaled dyes with emission from 720 to 835 nm

Technical Summary

CF®700
Abs/Em Maxima: 695/720 nm
Extinction coefficient: 240,000
Molecular weight: ~ 2315
Excitation laser line: 633, 635, 680 or 685 nm
Replaces: Alexa Fluor® 700, DyLight® 700

CF®750
Abs/Em Maxima: 755/777 nm
Extinction coefficient: 250,000
Molecular weight: ~ 3009
Excitation laser line: 633, 635, 680 or 685 nm
Replaces: Alexa Fluor® 750, Cy®7, DyLight® 750

CF®770
Abs/Em Maxima: 770/797 nm
Extinction coefficient: 220,000
Molecular weight: ~ 3138
Excitation laser line: 785 nm
Replaces: DyLight™ 800, IRDye 800CW

CF®790
Abs/Em Maxima: 784/806 nm
Extinction coefficient: 210,000
Molecular weight: ~ 3267
Excitation laser line: 785 nm
Replaces: Alexa Fluor® 790

CF®800
Abs/Em Maxima: 797/816 nm
Extinction coefficient: 210,000
Molecular weight: ~3334
Excitation laser line: 785 nm

CF®820
Abs/EmMaxima: 822/835 nm
Extinction coefficient: 253,000
Molecular weight: ~2553
Excitation laser line: 785 nm

Features
Exceptionally bright and stable
Ideal for in vivo imaging
Compatible with LI-COR® Odyssey®
Superior signal-to-noise for bioconjugates
Dye options for STORM & photoacoustic imaging

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    CF® Dyes for Multi-Color Super-Resolution Microscopy
Technical Summary
Features
CF Dyes validated in SIM, STORM, STED, TIRF, and more
Superior brightness, photostability, and photochemical switching
Single label antibody conjugates for STORM
Growing list of publications

View Technology

CF® dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared (NIR) spectrum for labeling biomolecules, especially proteins and nucleic acids. Developed by scientists at Biotium using new breakthrough chemistries, CF® dyes rival or exceed the quality of other dyes, such as Alexa Fluor® dyes.

Watch a video for a brief introduction to CF® dyes:

CF® dyes, now available with more than 30 bright and photostable fluorescent colors.
New Rhodamine Chemistry

 

Rhodamine dyes are known for their excellent photostability and good fluorescence quantum yield; consequently several of the Alexa Fluor® dyes bear the rhodamine core structure. Traditional rhodamine chemistry makes it difficult to extend the fluorescence wavelength to the far-red region and even more challenging in the near-IR region, especially for water-soluble dyes for bioconjugation.

However, Biotium scientists have discovered a new way to prepare novel rhodamine dyes of any fluorescence color from green to near-IR. The new chemistry is a key element in the development of many of our CF® dyes, particularly our far-red CF® dyes, which are not only bright and water-soluble but also extremely photostable.

Super-Resolution Microscopy

 

Recent publications comparing synthetic dyes for super-resolution imaging have shown CF® dyes give the best performance for multiple methods. The superior brightness, photostability, and photochemical switching properties of certain CF® dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® dyes for super-resolution microscopy.

Excellent Labeling Efficiency

 

Reactive dyes for bioconjugation are generally susceptible to hydrolysis, which can cause problems for shipping, handling and storage and result in lower labeling efficiency. Heavily sulfonated dyes, such as the Alexa Fluor® dyes, DyLight® dyes and IRDye® are particularly hygroscopic, worsening the hydrolysis problem.

For example, the percent of active Alexa Fluor® 488 succinimidyl ester (SE) could be well below 50% by the time of application (according to the Alexa Fluor® 488 microscale labeling kit product information sheet, Invitrogen). In contrast, all of Biotium’s amine-reactive CF® dyes have a relatively stable form of SE, which is more resistant to hydrolysis than the SE in many of the Alexa Fluor® dyes. Accordingly, CF® dye SE products generally give consistently higher labeling efficiency, thus providing users a better value.

Unrivaled near-IR dyes

 

Typical near-IR dyes are larger in size than dyes in the visible range, resulting in low dye solubility, dye aggregation and poor fluorescence quantum yield. To overcome these problems, many near-IR dyes, such as the near-IR Alexa Fluor® dyes, DyLight® dyes and IRDyes®, are engineered to have negatively charged sulfonate groups. While sulfonation improves dye solubility and fluorescence quantum yield to some degree, it creates another serious problem: non-specific binding of the bioconjugates prepared from the dyes caused by the highly negatively charged dyes.

Biotium scientists devised a revolutionary new approach to near-IR dye design that results in superior physical properties of the dyes without introducing an excessive amount of negative charge. Our near-IR CF® dyes are modified such that the intramolecular mobility of the dyes is restricted, which leads to higher quantum yield and better water solubility without adding excessive charge. As a result, near-IR CF® dyes are much brighter and more photostable than any other near-IR dyes. Most importantly, antibodies labeled with near-IR CF™ dyes give far better signal-to-noise ratio in immunostaining compared with antibody conjugates prepared with other commercial near-IR dyes. Learn more about IR CF® dyes.

Mix-n-Stain™ Antibody Labeling Technology

 

Biotium has developed a breakthrough antibody labeling technology with CF® dyes: Mix-n-Stain™ antibody labeling kits. With this technology, you merely need to mix your antibody with the reaction buffer and CF® dye provided in the kit, and in 30 minutes you will have an optimally labeled CF® dye-antibody conjugate ready for immunostaining.Mix-n-Stain™ provides unprecedented convenience for multicolor immunostaining, where pre-labeled primary antibodies may not be available while indirect staining via prelabeled fluorescent secondary antibodies may also be difficult due to cross-interaction among different antibodies.

Multi-Color Flexibility

 

Biotium currently offers 30 CF® dyes spanning the visible, far-red, and near-infrared spectra, with additional colors in development. The CF® dye product line includes reactive dyes, secondary antibodies, streptavidin, phallodin and other conjugates, and antibody labeling kits. Download the CF® Dye Brochure to see how CF® dyes compare to the dyes you’re using, and to see a comprehensive list of CF® dye products.

 

Please click on the download button below to order from a wide of range of CF dyes. Please email the form to sales@atlantisbioscience.com