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After testing a panel of lipofuscin quenching methods, including quenching with cupric sulfate, bleaching in Dent’s fixative, detergent extraction, Murray’s clearing, treatment with 8% SDS, and standard Sudan Black blocking, Biotium’s TrueBlack® Lipofuscin Autofluorescence Quencher was determined to be the only acceptable option for overcoming lipofuscin autofluorescence without causing V3HCR signal loss.

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Gürhan Özkayar, Ege Mutlu, Şebnem Şahin, Yağmur Demircan Demircan Yalçın, Taylan Töral, Haluk Külah, Ender Yıldırım, Özge Zorlu, Ebru Özgür.A Novel Microfluidic Method Utilizing a Hydrofoil Structure to Improve Circulating Tumor Cell Enrichment: Design and Analytical Validation.Micromachines (Basel). 2020 Oct 30;11(11):981. doi: 10.3390/mi11110981

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