Efficient, easy-to-use kits for labeling antibodies and other proteins with our superior CF® dyes and other labels.
Jump to a section below to choose a kit that’s right for you:

Easy-to-use with minimal hands-on time, Mix-n-Stain™ kits require no purification after labeling

Antibody labeling for flow cytometry made easy

Make your own enzyme-coupled antibodies

Create fluorescent SNAP-tag®, CLIP-tag™, Halotag® & other ligands, no purification required

Larger scale kits, for optimized protein labeling & purification

Kits for labeling antibodies with our superior near-IR CF® Dyes, for in vivo small animal imaging

MIX-N-STAIN™ CF® DYE ANTIBODY LABELING TECHNOLOGY

Mix-n-Stain™ antibody labeling kits dramatically simplify the process of antibody labeling. The resulting conjugates perform comparably to pre-labeled antibodies from leading suppliers. Because the labeling is covalent, the conjugates are stable for long-term storage, and ideal for multi-color imaging and multiplex flow cytometry.

Each Mix-n-Stain™ kit comes with everything you need to perform the conjugation. Without a separation step, you will have an optimally labeled antibody conjugate ready to be used in any immunofluorescence staining experiment.

One breakthrough feature of Mix-n-Stain™ kits is that antibody labeling can be conducted in the presence of many common buffer components such as BSA, gelatin, azide and low concentrations of Tris. As a result, the kits are compatible with most commercial antibody formulations without an antibody purification step prior to the conjugation process.

See below for our selection of Mix-n-Stain™ kits with a variety of label options.

Rapid Antibody Labeling with Next-Generation Fluorescent CF® Dyes, Biotin, & More

With Mix-n-Stain™ CF® Dye Antibody Labeling Kits, you can covalently label as little as ≤5 ug up to 100 ug of an antibody in just 30 minutes, with minimal hands-on time and 100% recovery of antibody. With slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid, for unrivaled convenience. See the Compatibility and Protocol Selection Flowchart below, or download the Product Protocol for a step-by-step worksheet to determine kit compatibility with your antibody.

These kits feature a choice of 29 superior CF® dyes, which have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, or other fluorescent dyes. Due to the high water solubility of CF® dyes, other proteins like BSA or gelatin in the labeling reaction have minimal effect on non-specific background, because non-IgG proteins are washed away during IF staining. We also offer Mix-n-Stain™ kits for biotin, FITC, DIG, and DNP labeling, as well as Mix-n-Stain™ Maxi Antibody Labeling Kits for 1 mg scale labeling reactions.

Features:

  • 3 kit sizes: 5-20 ug, 20-50 ug, or 50-100 ug Ab
  • Choice of 29 CF® Dye colors, biotin, FITC, or haptens
  • Less than 30 seconds of hands-on time
  • 30 minutes total reaction time
  • No purification of the product is required
  • Compatible with common antibody stabilizers

Compatible with antibody stabilizers and ascites

Monoclonal mouse anti-tubulin clone DM1A in ascites or purified in PBS with 1% BSA was labeled with CF®488A Mix-n-Stain™ using the modified protocol for total protein labeling. The Mix-n-Stain™-labeled antibodies performed comparably in immunofluorescence staining of HeLa cells as unlabeled antibody (in ascites) detected with secondary antibody.

Combine direct & indirect IF for flexible multiplexing

Combined direct and indirect immunofluorescence staining of rat testis with mouse anti-tubulin and CF®488A goat anti-mouse (min x rat) (microtubules, green), CF®555 Mix-n-Stain™ labeled mouse anti-ZO1 (tight junctions, red) and CF®640R phalloidin (actin filaments, cyan). See our Tech Tip: Combined Direct & Indirect Immunofluorescence Using Primary Antibodies from the Same Host.

Featuring CF® Dyes with superior brightness

Mouse anti-transferrin receptor antibody from (endosome and plasma membrane marker) was labeled using Lightning-Link® Rapid DyLight® Conjugation Kits from Novus Biologicals (left) or Mix-n-Stain™ CF® Dye Antibody Labeling Kits (right) according to manufacturers’ instructions. The CF® dye conjugates show higher signal and more specific staining compared the DyLight®conjugates when imaged using the same settings, due to the superior brightness of CF® dyes. The insets show the same field of view imaged with a higher gain setting.

Mix-n-Stain CF® Dye, Biotin, FITC & Hapten Antibody Labeling Kits

Product description Label options Labeling time
Mix-n-Stain™ CF® Dye Antibody Labeling Kits Choice of 29 bright & photostable CF® Dyes 30 minutes (~30 seconds hands-on time)

 

Download the product protocol

Mix-n-Stain™ Nanobody Labeling Kits Choice of 7 bright & photostable CF® Dyes and Biotin  
Mix-n-Stain™ FITC Antibody Labeling Kits FITC  
Mix-n-Stain™ Biotin Antibody Labeling Kits Biotin  
Mix-n-Stain™ Digoxigenin Antibody Labeling Kits Digoxigenin (DIG)  
Mix-n-Stain™ DNP Antibody Labeling Kits DNP  

Please click on the download button below to order from a wide of range of Mix-n-Stain Antibody Labeling Kits. Please email the form to sales@atlantisbioscience.com

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MIX-N-STAIN™ NANOBODY LABELING KITS

Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).

Features

  • Label 5-20 ug or 20-50 ug of Nanobody®
  • Choice of 7 CF® Dye colors or biotin
  • Simple 30 minute labeling, no purification
  • Compatible with common stabilizers
  • Dye options for super-resolution and 2-photon imaging (learn more)
Anti-histone nanobody was labeled using the Mix-n-Stain™ CF®488A 5-20 ug Nanobody Labeling Kit, then used to stain methanol-fixed HeLa cells at 2 ug/mL. Labeling either 5 ug or 20 ug of nanobody generated bright and specific conjugates, but protein amounts at the upper end of the kit range were more optimal. Adding 15 ug BSA to 5 ug nanobody to bring the protein amount to 20 ug produced more optimal labeling and brighter staining. Labeled BSA did not affect specificity of staining.
Overview of the Mix-n-Stain™ Nanobody Labeling Protocol.

Mix-n-Stain™ Nanobody Labeling Kits

Label Ex/Em Labeling size
(ug nanobody)
Catalog number
Biotin N/A 5-20 ug 92500
20-50 ug 92501
CF®405S 404/431 nm 5-20 ug 92502
20-50 ug 92503
CF®488A 490/515 nm 5-20 ug 92504
20-50 ug 92505
CF®568 562/583 nm 5-20 ug 92506
20-50 ug 92507
CF®594 593/614 nm 5-20 ug 92508
20-50 ug 92509
CF®640R 642/662 nm 5-20 ug 92510
20-50 ug 92511
CF®647 650/665 nm 5-20 ug 92512
20-50 ug 92513
CF®680R 680/701 nm 5-20 ug 92514
20-50 ug 92515

Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.

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MIX-N-STAIN™ PE, APC, PERCP, & TANDEM DYE ANTIBODY LABELING KITS

R-PE (commonly called PE), APC and PerCP are fluorescent proteins widely used in flow cytometry. These fluorophores belong to the family of phycobiliproteins, which are derived from cyanobacteria and eukaryotic algae. Because of their high extinction coefficients and quantum yields, they are the brightest flow cytometry dyes in their respective channels. They also can be coupled to small molecule dyes to form long Stokes shift tandem dyes, which expand multiplexing capability. Traditional protein-protein conjugation is complicated and time-consuming, but Mix-n-Stain™ kits greatly simplify the procedure, making it easy for any biologist to label their antibody for flow cytometry.

Features:

  • Label 25-50 ug, 50-100 ug, or 1 mg Ab
  • Labeling procedure of ~ 4 hours or less with no purification
  • Choice of 5 bright fluorescent labels
  • Novel RPE-CF®647T & APC-CF®750T tandem dyes for PE-Cy®5/APC-Cy®7 channels
  • Greatly simplifies antibody labeling for flow cytometry
Anti-mouse secondary antibody was labeled with APC using either the Mix-n-Stain™ APC Labeling Kit, or a competitor’s APC labeling kit. The antibodies were used to detect mouse anti-CD3 staining of Jurkat cells. The Mix-n-Stain™ labeled antibody showed lower background without primary antibody (pink) and higher signal on CD3-labeled cells (blue).

Mix-n-Stain™ Fluorescent Protein Antibody Labeling Kits

Product
Name
Ex/Em Catalog
Number
Reaction
Size
Labeling
Time
Protocol
Mix-n-Stain™ R-PE
Antibody Labeling Kit
496, 546, 565/
578 nm
92298 25-50 ug IgG ~4 hours
(~15 min. hands-on)

PI-Mix-n-Stain™ R-PE
92299 50-100 ug IgG
Mix-n-Stain™ APC
Antibody Labeling Kit
650/660 nm 92306 25-50 ug IgG
PI-Mix-n-Stain™ APC
92307 50-100 ug IgG
Mix-n-Stain™ PerCP
Antibody Labeling Kit
482/677 nm 92308 25-50 ug IgG
PI-Mix-n-Stain™ PerCP
92309 50-100 ug IgG
Mix-n-Stain™ RPE-CF®647T
Antibody Labeling Kit
496/665
(PE-Cy®5 channel)
92340 25-50 ug IgG ~2 hours
(few min. hands-on)

PI-Mix-n-Stain™ RPE-CF®647
92341 50-100 ug IgG
92346 1 mg IgG
Mix-n-Stain™ APC-CF®750T
Antibody Labeling Kit
650/780 nm
(APC-Cy®7 channel)
92310 25-50 ug IgG
Mix-n-Stain™ APC-CF®750T Antibody Labeling Kit
92311 50-100 ug IgG

Note: Kits are not recommended for labeling IgM antibodies. Cy Dye is a registered trademark of GE Healthcare.

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MIX-N-STAIN™ HRP, ALKALINE PHOSPHATASE, and GOX ANTIBODY LABELING KITS

Mix-n-Stain™ Enzyme Antibody Labeling Kits allow you to prepare antibody conjugates labeled with horseradish peroxidase (HRP), alkaline phosphatase (AP), or glucose oxidase (GOx) in about 2-3 hours. The resulting labeled antibodies are suitable for use in applications such as western blotting, ELISA and immunohistochemistry.

Features:

  • Reaction sizes: 25-50 ug or 50-100 ug Ab (plus 10-20 ug for HRP)
  • Label with HRP, alkaline phosphatase (AP), or glucose oxidase (GOx)
  • 2-3 hour labeling, with no purification
  • Compatible with western blotting, ELISA and immunohistochemistry

Mix-n-Stain™ Enzyme Antibody Labeling Kits

Product description Catalog number Reaction size Product protocol Labeling time
Mix-n-Stain™ HRP Antibody Labeling Kit 92300 10-20 ug antibody
PI-Mix-n-Stain™ HRP
~3.5 hours (few minutes hands-on time)
92301 25-50 ug antibody
92302 50-100 ug antibody
Mix-n-Stain™ Alkaline Phosphatase Antibody Labeling Kit 92314 25-50 ug antibody
PI-Mix-n-Stain™ AP
~2 hours (few minutes hands-on time)
92315 50-100 ug antibody
Mix-n-Stain™ Glucose Oxidase Antibody Labeling Kit 92312 25-50 ug antibody
PI-Mix-n-Stain™ GOx
92313 50-100 ug antibody

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MIX-N-STAIN™ CF® DYE SMALL LIGAND LABELING KITS

Mix-n-Stain™ CF® dye small ligand labeling kits are designed for rapid labeling of small (MW ~ 150 – 5,000) and relatively high affinity biological ligands (or substrates). Labeling takes about 30 minutes, without a final purification step. The ligands to be labeled must contain an aliphatic amine group that is not required for biological activity of the ligand. The amine group will form a covalent linkage with the reactive CF® dye provided in the kit. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands with an aliphatic amine. Many other small ligands are also possible candidates if they meet the criteria described above.

Simply mix your small molecule ligand with the reaction buffer and the optimally formulated dye provided, and incubate for 30 minutes, followed by a brief 5 minute quenching step. No reactive Mix-n-Stain™ dye is available at the end of labeling; therefore the conjugate is ready for cell staining and imaging without further purification. Mix-n-Stain™ labeled ligands perform comparably to commercially-available pre-labeled ligands (Figure 1), at a fraction of the cost.

For live cell imaging, the Mix-n-Stain™ small ligand kits are ideal to generate fluorescent chemical tags that provide smaller, brighter, and more versatile alternatives to fluorescent proteins. Labeling of various chemical tags with Mix-n-Stain™ kits yielded highly efficient and specific labeling of the target proteins both on surface and inside of living cells. The Mix-n-Stain™ small ligand kits have also been proven compatible with fixed cell imaging and gel-based assays.

Features:

  • Choice of 10 CF® Dye colors for extracellular staining
  • Choice of 5 CF® Dye colors for intracellular staining
  • For labeling molecules with a MW of 150-5000, containing an inactive aliphatic amine
  • Suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligands
  • 30 minutes total reaction time, no purification necessary
Figure 1. Live cell imaging of HeLa cells expressing CLIP-NK1R labeled with (A) CLIPamine conjugated to green fluorescent CF®488A using Mix-n-Stain™ compared to (B) CLIP-surface 488 from New England BioLabs. Cell nuclei were stained blue with Hoechst 33342.
Figure 2. Three color imaging in live HeLa cells. Cell surface protein ADRβ2 was labeled using the CF®488A Mix-n-Stain™ kit via the SNAP-tag® (green). Mitochondrial protein Cox8A was labeled using the CF®540 Mix-n-Stain™ kit via the CLIP-tag™ (red). Cell nuclei were stained blue with Hoechst 33342.

Mix-n-Stain Small Ligand Kits

Product description Label options Labeling time
Mix-n-Stain™ CF® Dye Small Molecule Labeling Kits Extracellular Staining: Choice of 10 bright and stable CF® Dyes ~30 minutes (minimal hands-on time)

Download the product protocol

Intracellular Staining: Choice of 5 bright and stable CF® Dyes

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CF® DYE SE
PROTEIN LABELING KITS

CF® Dye SE Protein Labeling Kits allow you to label your antibody of interest with one of our many bright and photostable CF® Dyes. The difference is that while the Mix-n-Stain™ kits come optimized for consistent labeling of small amounts of antibody, the SE Protein Labeling Kits are designed for 3 labelings of up to 1 mg protein with removal of free dye after labeling. The SE Protein Labeling Kits allow the user to optimize the amount of dye and antibody and determine the degree of protein labeling, for optimization of labeling and antibody performance.

Features:

  • Choice of 19 CF® Dye colors or biotin
  • Reagents for 3 labeling reactions with purification
  • Each reaction can label up to 1 mg of antibody or protein
  • Includes instructions for determining degree of labeling

VIVOBRITE™ NEAR-IR ANTIBODY LABELING KITS FOR IN VIVO IMAGING

VivoBrite™ Antibody Labeling Kits use the same labeling technology as our SE Protein Labeling Kits. The kits include the same components as the SE Protein Labeling Kits, with the addition of a syringe and syringe filter to sterile filter the labeled protein, allowing it to be used for in vivo small animal studies. The VivoBrite™ kits are offered in 4 near-IR CF® Dye colors, which are useful for small animal in vivo imaging. Biotium’s near-IR fluroescent dyes are best in class for brightness, photostability, and water solubility.

Features:

  • Choice of 4 near-IR CF® Dye colors
  • Kit contains reagents for 3 labeling reactions
  • Each reaction can label up to 1 mg of antibody
  • Kit can also be used to label other proteins
  • The resulting labeled protein is purified and sterile

Protein Labeling Kits

Product descriptionLabel optionsProduct protocol
CF® Dye SE Protein Labeling Kits19 CF® Dye colors across the spectrum, plus biotinPI-CF® Dye SE Protein Labeling Kits
VivoBrite™ Antibody Labeling Kits for Small Animal In Vivo Imaging4 near-IR CF® DyesPI-VivoBrite™ Antibody Labeling Kits

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Alexa Fluor is a registered trademark of Thermo Fisher Scientific. Cy is a registered trademark of GE Healthcare. SNAP-tag is a registered trademark of New England Biolabs. CLIP-tag is a trademark of New England Biolabs. HaloTag is a registered trademark of Promega.